Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

16-316 Anti-Phosphotyrosine Antibody, 4G10® Platinum, HRP Conjugate

16-316
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AWB, ELISAMHRPMonoclonal Antibody
Description
Catalogue Number16-316
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Phosphotyrosine Antibody, 4G10® Platinum, HRP Conjugate
Alternate Names
  • phosphotyrosine
Background InformationThe development of the anti-phosphotyrosine, clone 4G10® in 1989 was a monumental discovery for researchers. 4G10® was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10® is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10® with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10® Platinum. PY20 itself is a very poor substitute for 4G10®, but its additive effect allow for a greater level of detection on more substrates that even 4G10® alone.
Product Information
FormatHRP
Presentation100 μL of a proprietary mixture of protein G purified mouse monoclonal 4G10® (IgG2bƙ) HRP conjugate and protein A purified mouse monoclonal PY20, HRP conjugate (IgG2b) in PBS containing 0.05% Kathon® and HRP stabilizing compounds. Sufficient for a minumum of 10 immunoblots, if used at the optimum concentration of 1:2000 – 1:4000 in 10 mL blocking buffer. Liquid.
Quality LevelMQ100
Applications
ApplicationDetect Phosphotyrosine with Anti-Phosphotyrosine Antibody, 4G10 Platinum, HRP Conjugate (Mouse Monoclonal Antibody), that has been shown to work in WB, ELISA.
Key Applications
  • Western Blotting
  • ELISA
Biological Information
Immunogen4G10® used Phosphotyramine coupled to KLH and PY20 used phosphotyrosine conjugated to carrier protein
Clone4G10® and PY20
HostMouse
SpecificityTyrosine-phosphorylated proteins from all species.
IsotypeIgG2b
Species Reactivity
  • All
Antibody TypeMonoclonal Antibody
Modifications
  • Phosphorylation
Purification MethodProtein A Purfied
Molecular WeightVaries
Product Usage Statements
Quality AssuranceRoutinely evaluated by western blot.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
16-316 04053252729423