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FCMAB113A4 Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor® 488 conjugate

Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor 488 conjugate validated for use in FC & IC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Pluripotent & Early Differentiation Gene Symbol: MGC22487, OCT3, OCT4, OTF3, OTF4, OTTHUMP00000029292, Oct-3, Oct-4 , Oct4 UniProt Number: Q01860
FCMAB113A4
100 tests  
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Overview

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, ICCMAlexaFluor®488Monoclonal Antibody
Description
Catalogue NumberFCMAB113A4
DescriptionAnti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor® 488 conjugate
Alternate Names
  • Octamer-binding transcription factor 3
  • POU class 5 homeobox 1
  • POU domain class 5, transcription factor 1
  • POU domain, class 5, transcription factor 1
  • POU-type homeodomain-containing DNA-binding protein
  • octamer-binding transcription factor-3
Background InformationOctamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent embryonic stem (ES) and embryonic carcinoma (EC) cells express Oct-4. Additionally, murine embryonic germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
References
Product Information
FormatAlexaFluor®488
Control
  • Human embryonic stem cells
PresentationPurified mouse monoclonal IgG1 conjugated to Alexa Flour® 488 in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.
Quality LevelMQ100
Flow Cytometry:
Antibody dilution for cellular staining:
• Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.
• Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
• 5 µL for Guava Flow Cytometer
• 10 µL for other Flow Cytometry instruments
Applications
ApplicationDetect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor 488 conjugate validated for use in FC & IC.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
Working Solution Cells / test Total Reaction Volume
5 μl 5 x 105 Cells / 95 μl PBS 100 μl
10 μl 1 x 106 Cells / 90 μl PBS 100 μl
Biological Information
ImmunogenRecombinant GST fusion protein, human Oct-4 (aa 1-134).
Clone10H11.2
HostMouse
SpecificityReacts with human Oct-4.
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MGC22487
  • OCT3
  • OCT4
  • OTF3
  • OTF4
  • OTTHUMP00000029292
  • Oct-3
  • Oct-4
  • Oct4
UniProt Number
UniProt SummaryFUNCTION:Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency By similarity.
SUBUNIT STRUCTURE:Interacts with UBE2I By similarity. Interacts with PKM2.
SUBCELLULAR LOCATION: Nucleus. Note: Expressed in a diffuse and slightly punctuate pattern.
TISSUE SPECIFICITY: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
INDUCTION: Transcriptional activity is positively regulated by PKM2.
DOMAIN: The POU-specific domain mediates interaction with PKM2.
PTM: Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression By similarity.
MISCELLANEOUS: Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis.
SEQUENCE SIMILARITIES:Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.Contains 1 POU-specific domain.
Molecular Weight~44 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in 2102Ep Cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt
Packaging Information
Material Size100 tests
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
FCMAB113A4 04053252623707

Documentation

Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor® 488 conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor® 488 conjugate Certificates of Analysis

TitleLot Number
Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - NG1741716 NG1741716
Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - NRG1675588 NRG1675588
Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 1970353 1970353
Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 1990429 1990429
Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 2063186 2063186
Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 2110542 2110542
Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 2196060 2196060
Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 2227191 2227191
Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - NG1800880 NG1800880
Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - NG1832963 NG1832963
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References

Reference overviewPub Med ID
Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions.
Beers, J; Gulbranson, DR; George, N; Siniscalchi, LI; Jones, J; Thomson, JA; Chen, G
Nature protocols  7  2029-40  2012

Show Abstract 		23099485 23099485

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