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482728 Anti-Nitric Oxide Synthase (1131-1144), Inducible Rabbit pAb

This Anti-Nitric Oxide Synthase, Inducible Rabbit pAb is validated for use in Frozen Sections, WB, ICC, Paraffin Sections for the detection of Nitric Oxide Synthase, Inducible.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-iNOS

482728
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
482728-100UL Plastic ampoule 100 ul
Description
OverviewRecognizes the ~130 kDa iNOS protein.
Catalogue Number482728
Brand Family Calbiochem®
SynonymsAnti-iNOS
Application Data
Detection of mouse inducible nitric oxide synthase by immunoblotting. Sample: Whole cell lysate from RAW 264.7 cells induced with IFNγ and LPS. Primary antibody: Anti-Nitric Oxide Synthase (1131-1144), Inducible Rabbit pAb (Cat. No. 482728) (1:1000). Detection: chemiluminescence.
References
ReferencesLowenstein, C., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 6711.
Lyons, C., et al. 1992. J. Biol. Chem. 267, 6370.
Bredt, D.S., et al. 1991. Nature 351, 714.
Product Information
FormLiquid
FormulationUndiluted serum.
Positive controlRaw 264.7 cells treated with IFNγ and LPS
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesFrozen Sections (1:500)
Immunoblotting (1:2000)
Immunocytochemistry (1:500)
Paraffin Sections (1:500)
Application CommentsExhibits no cross-reactivity with bNOS or eNOS. Variables associated with assay conditions will dictate the proper working dilution.

Recommended Procedure for Immunocytochemistry of Anti-iNOS:
1. Grow cells in appropriate medium.
2. Induce NOS by pretreating cells for 16 h with fresh media containing either: no addition (control), interferon-γ (50 ng/ml), LPS (30 µg/ml) or interferon plus LPS.
3. Wash cells 3-5 min with phosphate buffered saline (PBS).
4. Permeabilize with 2% saponin for 10 min at room temperature.
5. Fix in 4% formalin for 10 min at room temperature.
6. Wash 3-5 min with PBS.
7. Incubate for 20 min at room temperature with anti-iNOS diluted 1:500 in PBS.
8. Wash 3-5 min with PBS.
9. Incubate with biotinylated secondary antibody (i.e. biotin-conjugated goat anti-rabbit IgG, Cat. No. 401313). Wash; incubate with avidin-conjugated alkaline phosphatase (Cat. No. 189726 or 189732).
10. Develop with Naphthol AS Phosphate/Fast Red Violet LB (Cat. No. 477758) or Naphthol AS Phosphate/New Fuchsin (Cat. No. 477759).
Biological Information
Immunogena synthetic peptide (CKKGSALEERKATRL) corresponding to amino acids 1131-1144 of mouse macrophage inducible nitric oxide synthase (iNOS), conjugated to KLH
ImmunogenMouse
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
482728-100UL 07790788050726

Documentation

Anti-Nitric Oxide Synthase (1131-1144), Inducible Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Nitric Oxide Synthase (1131-1144), Inducible Rabbit pAb Certificates of Analysis

TitleLot Number
482728

References

Reference overview
Lowenstein, C., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 6711.
Lyons, C., et al. 1992. J. Biol. Chem. 267, 6370.
Bredt, D.S., et al. 1991. Nature 351, 714.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-August-2007 RFH
SynonymsAnti-iNOS
ApplicationFrozen Sections (1:500)
Immunoblotting (1:2000)
Immunocytochemistry (1:500)
Paraffin Sections (1:500)
Application Data
Detection of mouse inducible nitric oxide synthase by immunoblotting. Sample: Whole cell lysate from RAW 264.7 cells induced with IFNγ and LPS. Primary antibody: Anti-Nitric Oxide Synthase (1131-1144), Inducible Rabbit pAb (Cat. No. 482728) (1:1000). Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~130 kDa inducible nitric oxide synthase (iNOS) protein.
HostRabbit
Immunogen speciesMouse
Immunogena synthetic peptide (CKKGSALEERKATRL) corresponding to amino acids 1131-1144 of mouse macrophage inducible nitric oxide synthase (iNOS), conjugated to KLH
IsotypeIgG
Specieshuman, mouse, rat
Positive controlRaw 264.7 cells treated with IFNγ and LPS
FormLiquid
FormulationUndiluted serum.
Preservative≤0.1% sodium azide
CommentsExhibits no cross-reactivity with bNOS or eNOS. Variables associated with assay conditions will dictate the proper working dilution.

Recommended Procedure for Immunocytochemistry of Anti-iNOS:
1. Grow cells in appropriate medium.
2. Induce NOS by pretreating cells for 16 h with fresh media containing either: no addition (control), interferon-γ (50 ng/ml), LPS (30 µg/ml) or interferon plus LPS.
3. Wash cells 3-5 min with phosphate buffered saline (PBS).
4. Permeabilize with 2% saponin for 10 min at room temperature.
5. Fix in 4% formalin for 10 min at room temperature.
6. Wash 3-5 min with PBS.
7. Incubate for 20 min at room temperature with anti-iNOS diluted 1:500 in PBS.
8. Wash 3-5 min with PBS.
9. Incubate with biotinylated secondary antibody (i.e. biotin-conjugated goat anti-rabbit IgG, Cat. No. 401313). Wash; incubate with avidin-conjugated alkaline phosphatase (Cat. No. 189726 or 189732).
10. Develop with Naphthol AS Phosphate/Fast Red Violet LB (Cat. No. 477758) or Naphthol AS Phosphate/New Fuchsin (Cat. No. 477759).
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesLowenstein, C., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 6711.
Lyons, C., et al. 1992. J. Biol. Chem. 267, 6370.
Bredt, D.S., et al. 1991. Nature 351, 714.