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MAB3329 Anti-MMP-14 Antibody, catalytic domain, clone LEM-2/63.1

MAB3329
100 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MELISA, IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB3329
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-MMP-14 Antibody, catalytic domain, clone LEM-2/63.1
Alternate Names
  • MT1-MMP
Background InformationMT1-MMP plays an important role during endothelial cell migration and matrix remodeling. Although the role of MT1-MMP in endothelial cell motility is not fully characterized, its activity appears to modulate endothelial migration, invasion, and formation of capillary tubes during the angiogenic response (Galvez, 2001). MT1-MMP also appears to play a key role in monocyte revruitment during inflammation.
Product Information
FormatPurified
PresentationPurified immunoglobulin by Protein A chromatography . Liquid in 0.2M phosphate, 0.25M NaCl, pH 7.6, containing 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-MMP-14 Antibody, catalytic domain, clone LEM-2/63.1 detects level of MMP-14 & has been published & validated for use in ELISA, IP & WB.
Key Applications
  • ELISA
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern Blot

Immunohistochemistry: Frozen sections

Immunofluorescence

Immunoprecipitation

Flow Cytometry

Blocking

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenSynthetic peptide: amino acid sequence 218-233 within the catalytic domain
Epitopecatalytic domain
CloneLEM-2/63.1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityLEM-2/63.1 reacts with human MT1-MMP and displays crossreactivity with mouse specimens. This antibody was generated against the catalytic domain of MT1-MMP and is able to inhibit enzyme activity.
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryProteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion.
Gene Symbol
  • MMP14
  • MTMMP1
  • MMP-14
  • MT1MMP
  • MT1-MMP
  • MMP-X1
  • EC 3.4.24.80
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P50281 # Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface.
COFACTOR: Binds 1 zinc ion per subunit (By similarity). & Calcium (By similarity).
SIZE: 582 amino acids; 65884 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
TISSUE SPECIFICITY: In stromal cells of colon, breast, and head and neck.
DOMAIN: SwissProt: P50281 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2° to 8°C for up to 12 months from date of receipt.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
MAB3329 04053252724558