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MAB3418A5 Anti-MAP2 Antibody, clone AP20, Alexa Fluor® 555 Conjugate

This Anti-MAP2 Antibody, clone AP20, Alexa Fluor 555 Conjugate is validated for use in ICC for the detection of MAP2.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: Map2, Mtap2 UniProt Number: F1MEW3
MAB3418A5
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, Ch, H, M, Qu, R, XnICCMAlexaFluor®555Monoclonal Antibody
Description
Catalogue NumberMAB3418A5
DescriptionAnti-MAP2 Antibody, clone AP20, Alexa Fluor® 555 Conjugate
Alternate Names
  • Microtubule-associated protein 2
  • MAP-2
  • MAP2
Background InformationThe microtubule-associated protein 2 (MAP-2) is an abundant neuronal cytoskeletal protein that binds to tubulin, stabilizes microtubules (Herzog and Weber, 1978), and is an excellent marker for neurons. MAP-2 is essential for the development and maintenance of neuronal morphology (Matus, 1991). Three primary isoforms of MAP-2, the high molecular weight MAP2a and MAP2b, and the low molecular weight MAP2c (resulting from alternate splicing of the MAP2 gene), have been identified in neurons. (Chung et al., 1996). The low molecular weight isoform, MAP2c, is expressed in developing brain and is down-regulated during brain maturation, whereas the high molecular weight MAP2b is expressed in both developing and adult brain. The MAP2a appears only after brain maturation (Tucker, 1990). All these forms bind to microtubules through a domain near the carboxyl terminus that contains either three or four similar repeats of a 31-amino-acid motif (Lewis et al., 1988). MAP-2 together with MAP-4 and tau proteins belong to the family of thermostable proteins associated with microtubules.
Product Information
FormatAlexaFluor®555
Control
  • Rat E18 primary cortex cells
PresentationPurified mouse monoclonal IgG conjugated to Alexa Fluor® 555 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality LevelMQ100
Applications
ApplicationThis Anti-MAP2 Antibody, clone AP20, Alexa Fluor 555 Conjugate is validated for use in ICC for the detection of MAP2.
Key Applications
  • Immunocytochemistry
Biological Information
ImmunogenBovine brain microtubule protein.
CloneAP20
HostMouse
IsotypeIgG
Species Reactivity
  • Bovine
  • Chicken
  • Human
  • Mouse
  • Quail
  • Rat
  • Xenopus
Species Reactivity NoteBovine, Chicken, Human, Mouse, Quail, Rat, Xenopus.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described.
Gene Symbol
  • Map2
  • Mtap2
Purification MethodAffinity purfied.
UniProt Number
UniProt SummaryFunction The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.



Subcellular location Cytoplasm › cytoskeleton Probable.



Post-translational modification Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly By similarity. MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR. Isoform MAP2c is phosphorylated by FYN at Tyr-67. Ref.9 Ref.10 Ref.11 Ref.12 Ref.13



Sequence similarities Contains 3 Tau/MAP repeats.
Molecular Weight~230 kDa observed.
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in rat E18 primary cortex cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected MAP2 in rat E18 primary cortex cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
MAB3418A5 04053252689000