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ABE1068 Anti-Lin-28B Antibody

Anti-Lin-28B is an antibody against Lin-28B for use in WB, IH.

Anti-Lin-28B Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: LIN28B, CSDD2
 UniProt Number: Q6ZN17
ABE1068
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1068
DescriptionAnti-Lin-28B Antibody
Alternate Names
  • Protein lin-28 homolog B
  • Lin-28B
Background InformationLin28 is a developmentally regulated RNA binding protein (RBP). It’s down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells (Viswanathan, 2008). Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancers (Viswanathan, 2008). The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance (Wu, 2005). Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Lin-28B is an antibody against Lin-28B for use in WB, IH.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected Lin-28B in 10 µg of human testis tissue lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Lin-28B in human testis and human liver cancer sections tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human Lin-28B.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Bovine, Porcine based on 100% sequence homology. Other homologies: mouse, rat 83%.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • LIN28B
  • CSDD2
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~35/23 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Lin-28B in 10 µg of HepG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1068 04055977112573