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07-1534 Anti-JMJD3 Antibody (CT)

07-1534
100 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MICC, ELISA, WBRbPurifiedPolyclonal Antibody
Description
Catalogue Number07-1534
DescriptionAnti-JMJD3 Antibody (CT)
Alternate Names
  • JMJD
  • JmjC domain-containing protein 3
  • Jumonji domain-containing protein 3
  • Lysine demethylase 6B
  • jumonji domain containing 3
  • jumonji domain containing 3
  • histone lysine demethylase
Background InformationJMJD3 (Jumonji domain-containing protein 3) is part of the family of novel JmjC domain-containing histone demethylation (JHDM) enzymes. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. JMJD3 specifically demethylates Lys27 on histone H3, demethylating both the trimethylated and dimethylated form. JMJD3 plays a role in the regulation of posterior development by regulating HOX gene expression. It is also involved in the inflammatory response by regulating gene expression and macrophage differentiation
Product Information
FormatPurified
HS Code3002 15 90
Control
  • HeLa cells, NIH/3T3 cells
PresentationPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-JMJD3 Antibody (C-terminus) is a Rabbit Polyclonal Antibody for detection of JMJD3 Antibody (C-terminus) also known as JmjC domain-containing protein 3 & has been validated in ICC, ELISA & WB.
Key Applications
  • Immunocytochemistry
  • ELISA
  • Western Blotting
Application NotesWestern Blot Analysis:
This antibody is reported by an
outside laboratory to be suitable for Western blotting


ELISA:
This antibody is reported by an
outside laboratory to be suitable for ELISA
Biological Information
ImmunogenThis antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of murine JMJD3.
EpitopeC-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes the C-terminal region of murine JMJD3. Additional non-specific, lower molecular weight bands are detected in some lysates.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman and mouse. Not tested in other species.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • EC 1.14.11
  • KDM6B
  • KIAA0346
Purification MethodProtein G Chromatography
UniProt Number
UniProt SummaryFUNCTION: Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. Ref.5 Ref.6

Cofactor Ascorbate By similarity.

Fe(2+). Ref.5

SUBUNIT STRUCTURE: Interacts with TLE1 By similarity. Component of the MLL4 complex, at least composed of MLL4, ASH2L, RBBP5, WDR5, and JMJD3.

SUBCELLULAR lOCATION: NucleusProbable.

INDUCTION: By 12-O-tetradecanoylphorbol-13-acetate (TPA) in myeloid leukemia cells. Ref.4

SIMILARITY: Belongs to the UTX family.

Contains 1 JmjC domain.

Molecular Weight~180 kDa
Product Usage Statements
Quality AssuranceImmunocytochemisty:
Recognizes JMJD3 in NIH 3T3 and A431 cells by cytochemistry.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
07-1534 04053252583179