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MABE872 Anti-Histone H3.3 Antibody, clone 4H2D7

This Anti-Histone H3.3 Antibody, clone 4H2D7 is validated for use in western blotting, ICC & ChIP for the detection of Histone H3.3.

Anti-Histone H3.3 Antibody, clone 4H2D7 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: H3F3B, H3.3B UniProt Number: P84243
MABE872
200 µg  
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Overview

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICC, ChIPRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE872
DescriptionAnti-Histone H3.3 Antibody, clone 4H2D7
Alternate Names
  • Histone H3.3
  • H3F3A
  • H3.3A
  • H3F3
Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Histone H3.3 Antibody, clone 4H2D7 is validated for use in western blotting, ICC & ChIP for the detection of Histone H3.3.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Chromatin Immunoprecipitation (ChIP)
Application NotesImmunocytochemistry Analysis: A 1:50 dilution from a represenative lot detected Histone H3.3 in HeLa cells.

Western Blotting Analysis: A representative lot from an independent laboratory detected recombinant Histone H3.3 (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Histone H3.3 in C2C12 cell lysate (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Histone H3.3 in C2C12 cells (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Histone H3.3 from C2C12 cell lysate (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Histone H3.3.
Clone4H2D7
Concentration1 mg/mL
HostRat
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • H3F3B
  • H3.3B
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~15 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 2 µg/mL of this antibody detected Histone H3.3 in 10 µg of HeLa acid extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE872 04053252975684

Documentation

Anti-Histone H3.3 Antibody, clone 4H2D7 SDS

Title

Safety Data Sheet (SDS) 

Anti-Histone H3.3 Antibody, clone 4H2D7 Certificates of Analysis

TitleLot Number
Anti-Histone H3.3, clone 4H2D7 3074357
Anti-Histone H3.3, clone 4H2D7 - Q2426891 Q2426891
Anti-Histone H3.3, clone 4H2D7 - 3266911 3266911
Anti-Histone H3.3, clone 4H2D7 - 3526340 3526340
Anti-Histone H3.3, clone 4H2D7 - 3918947 3918947
Anti-Histone H3.3, clone 4H2D7 - 4076675 4076675
Anti-Histone H3.3, clone 4H2D7 - 4180340 4180340
Anti-Histone H3.3, clone 4H2D7 -2702174 2702174
Anti-Histone H3.3, clone 4H2D7 Monoclonal Antibody 3003756
Anti-Histone H3.3, clone 4H2D7 Monoclonal Antibody 3136013

References

Reference overviewApplicationSpeciesPub Med ID
Chd2 interacts with H3.3 to determine myogenic cell fate.
Harada, A; Okada, S; Konno, D; Odawara, J; Yoshimi, T; Yoshimura, S; Kumamaru, H; Saiwai, H; Tsubota, T; Kurumizaka, H; Akashi, K; Tachibana, T; Imbalzano, AN; Ohkawa, Y
EMBO J  31  2994-3007  2012

Show Abstract 		Western BlottingMouse22569126 22569126