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MAB4065 Anti-H-CAM Antibody, Pan, clone SFF-2

This Anti-H-CAM Antibody, Pan, clone SFF-2 is validated for use in FC, IC, IH, IH(P) for the detection of H-CAM.

Anti-H-CAM Antibody, Pan, clone SFF-2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: CD44, LHR, HUTCH-I, CDW44, CDw44, ECMR-III, Epican, MGC10468, CD44R, PGP-1, MDU2, CSPG8, Pgp1, IN, MUTCH-I, MIC4, MDU3, MC56, HCELL UniProt Number: P16070
MAB4065
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, ICC, IHC, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB4065
Replaces04-1123
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-H-CAM Antibody, Pan, clone SFF-2
Alternate Names
  • CD44
  • CD44std
  • CD44H
  • HCAM
Product Information
FormatPurified
Control
  • POSITIVE CONTROL: Lymph node (lymphocytes), skin (keratinocytes), or tonsil.
PresentationLiquid in PBS.
Quality LevelMQ100
Applications
ApplicationThis Anti-H-CAM Antibody, Pan, clone SFF-2 is validated for use in FC, IC, IH, IH(P) for the detection of H-CAM.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
Applications Not Recommended
  • Western Blotting
Application NotesImmunohistochemistry: 1 µg/mL; acetone fixed cryostat sections; 2 µg/mL following heat-induced epitope retrieval in 0.01 M citrate buffer, pH 6.0 (Banfalvi, 1999).
Immunofluorescence
Flow Cytometry: 1:20 as determined on U937 cells
Optimal working dilutions must be determined by end user.
Biological Information
EpitopePan
CloneSFF-2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificitySpecific for human standard CD44. Does not distinguish between splice variants (exons v3v10).
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
Gene Symbol
  • CD44
  • LHR
  • HUTCH-I
  • CDW44
  • CDw44
  • ECMR-III
  • Epican
  • MGC10468
  • CD44R
  • PGP-1
  • MDU2
  • CSPG8
  • Pgp1
  • IN
  • MUTCH-I
  • MIC4
  • MDU3
  • MC56
  • HCELL
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P16070 # Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
SIZE: 742 amino acids; 81554 Da
SUBUNIT: Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: An epithelial isoform (CD44E) is expressed by cells of epithelium and highly expressed by carcinomas. An hematopoietic isoform (CD44H) is expressed by cells of mesodermal origin. Expression is repressed in neuroblastoma cells.
PTM: Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. & N-glycosylated. & O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). & Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
SIMILARITY: SwissProt: P16070 ## Contains 1 Link domain.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsPrior to opening maintain at -20°C until expiration date. After opening maintain frozen at -20°C in undiluted aliquots until expiration date. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
MAB4065 04053252399428