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MAB3194 Anti-Glutathione: N-ethylmaleimide adduct Antibody, clone 8.1GSH

MAB3194
100 µg  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MaELISA, FC, ICC, IHC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB3194
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Glutathione: N-ethylmaleimide adduct Antibody, clone 8.1GSH
References
Product Information
FormatPurified
HS Code3002 15 90
PresentationLiquid in 0.02M PBS pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect the Glutathione: N-ethylmaleimide adduct protein using this Anti-Glutathione: N-ethylmaleimide adduct, clone 8.1GSH validated for use in ELISA, Flow, ICC, IHC & WB.
Key Applications
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blotting
Application NotesImmunohistochemistry: 1:100 (paraformaldehyde fixation)

Immunocytochemistry: 1:100 - 1:500 for assessment of intracellular GSH distribution.

ELISA: 1:100,000 - 1:200,000 to quantify GSH at >2 mM in biological specimens.

Flow cytometry: 1:100 - 1:1,000

Cells were fixed with cold 1% PFA for 5 min and vortexed into methanol (-20°C) containing 5mM NEM (10 min on dry ice) or 10 μM NEM (N-ethyl maleimide) for 30 min at RT.

Optimal working dilutions must be determined by end user.

Positive Control: Peripheral blood mononuclear cells (PBMC) treated with NEM
Biological Information
ImmunogenGS-NEM conjugated to KLH
Clone8.1GSH
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReactive with thiol-modified glutatione. Does not react with oxidized glutathione (GSSG) or glutathione (GSH) in mixed disulfide linkage with proteins. MAB3194 is specific to the glutathione:N-ethylmaleimide adduct; thus it is useful for quantification of reduced glutathione (GSH) after reaction with N-ethylmaleimide. For use in ELISA or flow cytometry, sample must first be reacted with NEM.
IsotypeIgG1
Species Reactivity
  • Mammals
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB3194 04053252265754

Documentation

Anti-Glutathione: N-ethylmaleimide adduct Antibody, clone 8.1GSH SDS

Title

Safety Data Sheet (SDS) 

Anti-Glutathione: N-ethylmaleimide adduct Antibody, clone 8.1GSH Certificates of Analysis

TitleLot Number
MOUSE ANTI-GLUTATHIONE: N-ETHYLMALEIMIDE ADDUCT 2472914
MOUSE ANTI-GLUTATHIONE: N-ETHYLMALEIMIDE ADDUCT (GS-NEM) MONOCLONAL ANTIBODY - 2383488 2383488
MOUSE ANTI-GLUTATHIONE: N-ETHYLMALEIMIDE ADDUCT (GS-NEM) - 2705084 2705084
MOUSE ANTI-GLUTATHIONE: N-ETHYLMALEIMIDE ADDUCT (GS-NEM) - 3070206 3070206
MOUSE ANTI-GLUTATHIONE: N-ETHYLMALEIMIDE ADDUCT (GS-NEM) - 3185616 3185616
MOUSE ANTI-GLUTATHIONE: N-ETHYLMALEIMIDE ADDUCT (GS-NEM) - 3463581 3463581
MOUSE ANTI-GLUTATHIONE: N-ETHYLMALEIMIDE ADDUCT (GS-NEM) - 3613045 3613045
MOUSE ANTI-GLUTATHIONE: N-ETHYLMALEIMIDE ADDUCT (GS-NEM) - 3729360 3729360
MOUSE ANTI-GLUTATHIONE: N-ETHYLMALEIMIDE ADDUCT (GS-NEM) - 3998964 3998964
MOUSE ANTI-GLUTATHIONE: N-ETHYLMALEIMIDE ADDUCT (GS-NEM) -2545378 2545378

Brochure

Title
Pathways and Biomarkers of Oxidative Stress

Technical Info

Title
White Paper- Modern Methods in Oxidative Stress Research

Data Sheet

Title
MOUSE ANTI-GLUTATHIONE: N-ETHYLMALEIMIDE ADDUCT (GS-NEM)