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AB5849 Anti-Glutamate Receptor 1 Antibody, phosphoSer 845

AB5849
150 µL  
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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5849
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Glutamate Receptor 1 Antibody, phosphoSer 845
Alternate Names
  • GluR1
Background InformationGlutamate receptor 1 (AMPA) belongs to the glutamate-gated ion channel and binds AMPA, glutamate and kainate. L-Glutamate is the major excitatory neurotransmitter in the mammalian CNS, acting through both ligand gated ion channels (ionotropic receptors; composed of subunits GluR1-4) and G-protein coupled (metabotropic) receptors. The AMPA glutamate receptors mediate fast synaptic transmission in the CNS.
Product Information
FormatAffinity Purified
Control
  • Human brain tissue
PresentationAffinity Purified immunoglobulin. Liquid in 10 mM HEPES, pH 7.5, with 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Quality LevelMQ100
Applications
ApplicationAnti-Glutamate Receptor 1 Antibody, phosphoSer 845 is an antibody against Glutamate Receptor 1 for use in WB.
Key Applications
  • Western Blotting
Application NotesWestern blot: 1:1,000

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenSynthetic peptide corresponding to amino acids surrounding the phosphoSer845 of rat GluR1.
EpitopephosphoSer 845
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes Glutamate Receptor 1 [GluR1], phosphoSe845. The antibody recognizes a protein of 100 kDa corresponding to GluR1 phosphorylated at Ser845.
Species Reactivity
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryGlutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Gene Symbol
  • GRIA1
  • GluR-K1
  • GLURA
  • HBGR1
  • GluR-A
  • GluR-1
  • GLUH1
  • GLUR1
  • MGC133252
Modifications
  • Phosphorylation
Purification MethodImmunoAffinity Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P42261 # Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
SIZE: 906 amino acids; 101506 Da
SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with DLG1 via its C-terminus. Interacts with CACNG2 and RASGRF2 (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Note=Interaction with CACNG2 promotes cell surface expression (By similarity).
TISSUE SPECIFICITY: Widely expressed in brain.
PTM: Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).
SIMILARITY: SwissProt: P42261 ## Belongs to the glutamate-gated ion channel (TC 1.A.10) family.
MISCELLANEOUS: The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.
Molecular Weight100 kDa
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size150 µL
Global Trade Item Number
Catalogue Number GTIN
AB5849 04053252478215