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16-234 Anti-FAK Antibody, clone 4.47, Alexa Fluor® 555 conjugate

Anti-FAK Antibody, clone 4.47, Alexa Fluor 555 conjugate detects level of FAK & has been published & validated for use in FC, WB & IC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Cytoskeletal Signaling Gene Symbol: PTK2, pp125FAK, FADK, FAK1, FAK UniProt Number: Q05397
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100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RFC, WB, ICCMAlexaFluor®555Monoclonal Antibody
Description
Catalogue Number16-234
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-FAK Antibody, clone 4.47, Alexa Fluor® 555 conjugate
Product Information
FormatAlexaFluor®555
Quality LevelMQ100
Applications
ApplicationAnti-FAK Antibody, clone 4.47, Alexa Fluor 555 conjugate detects level of FAK & has been published & validated for use in FC, WB & IC.
Key Applications
  • Flow Cytometry
  • Western Blotting
  • Immunocytochemistry
Biological Information
ImmunogenGST fusion protein corresponding to residues 1-423 of human FAK (focal adhesion kinase)
Cloneclone 4.47
HostMouse
Specificityp125FAK, does not cross react with Pyk-2
IsotypeIgG1
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined.
Gene Symbol
  • PTK2
  • pp125FAK
  • FADK
  • FAK1
  • FAK
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q05397 # Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
SIZE: 1052 amino acids; 119233 Da
SUBUNIT: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with SHB (By similarity). Interacts with TGFB1I1.
SUBCELLULAR LOCATION: Cell junction, focal adhesion; Cytoplasmic side. Note=Constituent of focal adhesions.
TISSUE SPECIFICITY: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
DOMAIN: SwissProt: Q05397 The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL (By similarity). & The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
PTM: Phosphorylated on 6 tyrosine residues upon activation.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain.
Molecular Weight125kDa
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunostaining in 3T3 cells
Usage Statement
  • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at 4°C from date of shipment
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
16-234 04053252470844