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MAB394-100UG Anti-Dopamine β Hydroxylase-SAP Antibody, clone 4F10.2

MAB394-100UG
100 µg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RILMSaporinMonoclonal Antibody
Description
Catalogue NumberMAB394-100UG
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Dopamine β Hydroxylase-SAP Antibody, clone 4F10.2
References
Product Information
FormatSaporin
PresentationSaporin Conjugated. Sterile liquid. Provided in phosphate buffered saline. Contains no preservative.
Quality LevelMQ100
Applications
ApplicationDetect Dopamine β Hydroxylase using this Anti-Dopamine β Hydroxylase-SAP Antibody, clone 4F10.2 validated for use in 0.
Key Applications
  • Immunolesioning
Application NotesImmunotoxin
Biological Information
Clone4F10.2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityDopamine b-HydroxylaseDBH is a single gene product that exists as a cytosolic enzyme, as a membrane protein and is secreted into the circulation (Lewis & Asnani, 1992). Because of its residence time as a plasma membrane-bound protein, it is able to be used as a targeting agent for neurons. The target neurons include postganglionic sympathetic neurons and all noradrenergic and adrenergic neurons of the central nervous system. As such anti-DBH-SAP can be used for the production of acute peripheral autonomic failure in a model for the human disease. It can also be used for the analysis of the behavioral effects of noradrenergic or adrenergic ablation. When injected intraventricularly, noradrenergic neurons of the locus coeruleus are eliminated (Wiley & Lappi, 1994). The antibody and immunotoxin undergo retrograde transport.

MAB308 is the unconjugated monoclonal.
IsotypeIgG1
Species Reactivity
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide.
Gene Symbol
  • DBH
  • DBM
  • EC 1.14.17.1 [Contains: Soluble dopamine beta-hydroxylase].
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P09172 # Conversion of dopamine to noradrenaline.
COFACTOR: Binds 1 PQQ per subunit. & Binds 2 copper ions per subunit.
SIZE: 617 amino acids; 69065 Da
SUBUNIT: Homotetramer composed of two non-covalently bound disulfide-linked dimers.
SUBCELLULAR LOCATION: Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen. & Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single- pass type II membrane protein (Potential).
DISEASE: SwissProt: P09172 # Defects in DBH are the cause of DBH deficiency [MIM:223360]; also called norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction.
SIMILARITY: SwissProt: P09172 ## Belongs to the copper type II ascorbate-dependent monooxygenase family. & Contains 1 DOMON domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsThe material should be stored at -80°C for up to six months under sterile conditions. Avoid repeated freeze-thaw cycles.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB394-100UG 04053252398179

Documentation

Anti-Dopamine β Hydroxylase-SAP Antibody, clone 4F10.2 SDS

Title

Safety Data Sheet (SDS) 

Anti-Dopamine β Hydroxylase-SAP Antibody, clone 4F10.2 Certificates of Analysis

TitleLot Number
ANTI-DOPAMINE #946;-HYDROXYLASE (DBH)-SAP, clone 4F10.2 - 2115581 2115581
ANTI-DOPAMINE β-HYDROXYLASE (DBH)-SAP, clone 4F10.2 - 3745496 3745496
ANTI-DOPAMINE β-HYDROXYLASE (DBH)-SAP, clone 4F10.2 -2626801 2626801

References

Reference overviewPub Med ID
Central noradrenergic lesioning using anti-DBH-saporin: anatomical findings.
Wrenn, C C, et al.
Brain Res., 740: 175-84 (1996)  1996

Show Abstract
8973812 8973812
Anti-dopamine beta-hydroxylase immunotoxin-induced sympathectomy in adult rats.
Picklo, M J, et al.
J. Pharmacol. Exp. Ther., 275: 1003-10 (1995)  1995

Show Abstract
7473126 7473126
Soluble and membrane-bound forms of dopamine beta-hydroxylase are encoded by the same mRNA.
Lewis, E J and Asnani, L P
J. Biol. Chem., 267: 494-500 (1992)  1992

Show Abstract
1730612 1730612

Data Sheet

Title
ANTI-DOPAMINE -HYDROXYLASE (DBH)-SAP, clone 4F10.2

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Catalogue Number Description
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Alternative Packsize

Catalogue Number Description
MAB394-250UG Anti-Dopamine β Hydroxylase-SAP Antibody, clone 4F10.2

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies