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MABF39-I Anti-DC-STAMP Antibody, clone 1A2

Anti-DC-STAMP Antibody, clone 1A2 is an antibody against DC-STAMP for use in Western Blotting, Flow Cytometry, Immunocytochemistry, Immunoprecipitation.

Anti-DC-STAMP Antibody, clone 1A2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Osteobiology Gene Symbol: DCSTAMP, TM7SF4 UniProt Number: Q9H295
MABF39-I
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWB, FC, ICC, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF39-I
DescriptionAnti-DC-STAMP Antibody, clone 1A2
Alternate Names
  • Dendritic cell-specific transmembrane protein
  • DC-STAMP
  • Dendrocyte-expressed seven transmembrane protein
  • FIND
  • hDC-STAMP
  • IL-four-induced protein
  • Transmembrane 7 superfamily member 4
Background InformationDendritic cell-specific transmembrane protein (UniProt Q9H295; also known as DC-STAMP, Dendrocyte-expressed seven transmembrane protein, FIND, hDC-STAMP, IL-four-induced protein, Transmembrane 7 superfamily member 4) is encoded by the DCSTAMP (also known as TM7SF4) gene (Gene ID 81501) in human. DC-STAMP is a six-transmembrane protein essential for cell-to-cell fusion to form multinucleated osteoclasts (OCs) during osteoclastogenesis. DC-STAMP expression is upregulated among osteoclast precursor (OCP) cells upon exposure to OC-promoting cytokines, such as receptor activator of nuclear factor-κB (NF-κB) ligand (RANKL), and Dcstamp-knockout (KO) mice have few multinucleated TRAP+ OCs and increased bone mass. On the other hand, DC-STAMP overexpression in transgenic (Tg) mice causes accelerated cell-to-cell fusion during OCP differentiation and enhanced bone resorption. DC-STAMP is a six-trasmembrane (a.a. 35-55, 58-78. 98-118, 210-230, 293-313, 377-397) protein, having both its N- and C-terminal ends exposed intracellularly (a.a. 1-34, 398-470). The C-terminal cytoplasmic tail of DC-STAMP contains an immunoreceptor tyrosine-based inhibitory motif or ITIM sequence (407-SFYPSV-412) that, when phosphorylated on the tyrosine residue, recruits SHP-1. DC-STAMP neutralizing antibody blocks OC formation in vitro and abolishes cellular DC-STAMP and SHP-1 tyrosine phosphorylation.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ antibody in PBS without preservatives.
Quality LevelMQ100
Applications
ApplicationAnti-DC-STAMP Antibody, clone 1A2 is an antibody against DC-STAMP for use in Western Blotting, Flow Cytometry, Immunocytochemistry, Immunoprecipitation.
Key Applications
  • Western Blotting
  • Flow Cytometry
  • Immunocytochemistry
  • Immunoprecipitation
Application NotesWestern Blotting Analysis: 4.0 µg/mL from a representative lot detected DC-STAMP in 10 µg of thymus lysates from wild-type, but not Dcstamp-knockout mice.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected DC-STAMP in thymus and spleen lysates from wild-type mice, and greatly reduced DC-STAMP in thymus and spleen lysates from Dcstamp-knockout mice (Courtesy of Grace Chiu, Ph.D., University of Rochester Medical Center, NY, USA).
Western Blotting Analysis: A representative lot detected the ~106 kDa dimeric and the ~53 kDa monomeric DC-STAMP band by Western blotting under non-denatured and denatured condition, respectively, following DC-STAMP immunoprecipitation using murine RAW 264.7 macrophage lysate (Mensah, K.A., et al. (2010). J. Cell. Physiol. 223(1):76-83).
Flow Cytometry Analysis: A representative lot detected DC-STAMP-positive lymphoctes and monocytes in purified human PBMCs (Chiu, Y.H., et al. (2012). J. Bone Miner. Res. 27(1):79-92).
Flow Cytometry Analysis: A representative lot detected DC-STAMP surface expression on murine RAW 264.7 macrophages and murine bone marrow-derived CD11b+ monocytes. DC-STAMP is expressed on osteoclast precursor (OCP) cells as a dimer, which is efficiently detected by flow cytometry using clone 1A2 (Mensah, K.A., et al. (2010). J. Cell. Physiol. 223(1):76-83).
Function Analysis: A representative lot inhibited RANKL & M-CSF treatment-induced osteoclasts (OC) formation in human PBMCs & monocytes cultures (Chiu, Y.H., et al. (2012). J. Bone Miner. Res. 27(1):79-92).
Function Analysis: A representative lot inhibited RANKL treatment-induced osteoclasts (OC) formation in RAW 264.7 and murine bone marrow macrophages cultures (Mensah, K.A., et al. (2010). J. Cell. Physiol. 223(1):76-83).
Immunohistochemistry Analysis: A representative lot detected DC-STAMP expressionon in multinucleated ‘osteoclast-like’ giant cells in human giant cell tumor of bone (Chiu, Y.H., et al. (2012). J. Bone Miner. Res. 27(1):79-92).
Immunocytochemistry Analysis: A representative lot detected DC-STAMP-positive human PBMCs using 10% NBF-fixed, paraffin-embedded cell preparation (Chiu, Y.H., et al. (2012). J. Bone Miner. Res. 27(1):79-92).
Immunocytochemistry Analysis: A representative lot detected differential DC-STAMP intracellular localizations by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% saponin-permeabilized murine bone marrow macrophages at different time points during osteoclastogenesis upon RANKL treatment (Mensah, K.A., et al. (2010). J. Cell. Physiol. 223(1):76-83).
Immunoprecipitation Analysis: A representative lot immunoprecipitated DC-STAMP from the lysates of human monocytes (Chiu, Y.H., et al. (2012). J. Bone Miner. Res. 27(1):79-92).
Immunoprecipitation Analysis: A representative lot immunoprecipitated DC-STAMP from the membrane extracts of murine RAW 264.7 macrophages (Mensah, K.A., et al. (2010). J. Cell. Physiol. 223(1):76-83).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding a sequence from the third extracellular domain of human DC-STAMP.
EpitopeThe third extracellular domain.
Clone1A2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 1A2 recognizes an epitope conserved between human and murine DC-STAMP in the third extracellular domain.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • DCSTAMP
  • TM7SF4
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~58 kDa observed. Target band size appears larger than the calculated molecular weights of 53.39 kDa (human) and 53.88 kDa (mouse) due to glycosylation. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse thymus lysate.

Western Blotting Analysis: 4.0 µg/mL of this antibody detected DC-STAMP in 10 µg of mouse thymus lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF39-I 04055977349580

Documentation

Anti-DC-STAMP Antibody, clone 1A2 SDS

Title

Safety Data Sheet (SDS) 

Anti-DC-STAMP Antibody, clone 1A2 Certificates of Analysis

TitleLot Number
Anti-DC-STAMP, clone 1A2 - 3174761 3174761
Anti-DC-STAMP, clone 1A2 - 3275489 3275489
Anti-DC-STAMP, clone 1A2 - 3419591 3419591
Anti-DC-STAMP, clone 1A2 - 3574350 3574350
Anti-DC-STAMP, clone 1A2 - 3825997 3825997
Anti-DC-STAMP, clone 1A2 - 3954781 3954781
Anti-DC-STAMP, clone 1A2 - 4061146 4061146
Anti-DC-STAMP, clone 1A2 - 4174544 4174544
Anti-DC-STAMP, clone 1A2 - 4222017 4222017
Anti-DC-STAMP, clone 1A2 -2691480 2691480
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