Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABT520 Anti-Cytokeratin 18, clone 4D11E4 Antibody

Anti-Cytokeratin 18, clone 4D11E4 Antibody is an antibody against Cytokeratin 18 for use in Western Blotting, Immunocytochemistry, ELISA.

Anti-Cytokeratin 18, clone 4D11E4 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: KRT18, CYK18, PIG46 UniProt Number: P05783
MABT520
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MAscitesMonoclonal Antibody
Description
Catalogue NumberMABT520
Replaces04-586
DescriptionAnti-Cytokeratin 18, clone 4D11E4 Antibody
Alternate Names
  • Keratin
  • type I cytoskeletal 18
  • Cell proliferation-inducing gene 46 protein
  • Cytokeratin-18
  • CK-18
  • Keratin-18
  • K18
  • Cytokeratin 18
Background InformationKeratin, type I cytoskeletal 18 (KRT18), also known as Cytokeratin-18 (CK-18), are intermediate filament phosphoglycoproteins that are expressed preferentially in glandular epithelia. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Together with actin microfilaments and microtubules, keratin filaments make up the cytoskeletons of vertebrate epithelial cells, forming alpha-helical coiled-coil dimers which associate laterally and end-to-end to form 10-nm diameter filaments.
References
Product Information
FormatAscites
PresentationMouse monoclonal IgG2bascitic fluid containing 0.03% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-Cytokeratin 18, clone 4D11E4 Antibody is an antibody against Cytokeratin 18 for use in Western Blotting, Immunocytochemistry, ELISA.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected Cytokeratin 18 in HeLa, NIH/3T3, A549, Jurkat, MCF-7, HepG2, A431, and K562 cell lysates.

Immunohistochemistry Analysis (IHC): A 1:200-1,000 dilution from a representative lot detected Cytokeratin 18 in human breast carcinoma, hepatocarcinoma, stomach cancer, and colon cancer tissues.
Biological Information
ImmunogenPurified recombinant fragment of human Cytokeratin 18 expressed in E. Coli. 
Clone4D11E4
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2b
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • KRT18
  • CYK18
  • PIG46
Purification MethodUnpurified
UniProt Number
Molecular Weight~62 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting using cell lysates.

Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected Cytokeratin 18 in HeLa, NIH/3T3, A549, Jurkat, MCF-7, HepG2, A431, and K562 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT520 04055977171501