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ABE1974 Anti-CHMP1B

Anti-CHMP1B, Cat. No. ABE1974, is a highly specific rabbit polyclonal antibody that targets charged multivesicular body protein 1b and has been tested in Western Blotting.

Anti-CHMP1B MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: CHMP1B, C18orf2 UniProt Number: Q7LBR1
ABE1974
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1974
DescriptionAnti-CHMP1B
Alternate Names
  • Charged multivesicular body protein 1b
  • CHMP1.5
  • Chromatin-modifying protein 1b
  • Vacuolar protein sorting-associated protein 46-2
  • Vps46-2
  • hVps46-2
Background InformationCharged multivesicular body protein 1b (UniProt: Q7LBR1; CHMP1.5, Chromatin-modifying protein 1b, CHMP1b, Vacuolar protein sorting-associated protein 46-2, Vps46-2, hVps46-2) is encoded by the CHMP1B (also known as C18orf2) gene (Gene ID: 57132) in human. CHMP1b is a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. It is widely expressed and is found in pancreas, kidney, skeletal muscle, liver, lung, placenta and the brain tissue. CHMP1b is a peripherally associated component of the endosomal sorting system that is required for transport complex III (ESCRT-III), which is involved in multi-vesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. CHMP1b is localized in cytosol, endosome, and in peripheral membrane protein. CHMP1b binds specifically to the AAA ATPase spastin and thereby recruits this microtubule severing protein to the midbody. CHMP1b and spastin are shown to colocalize at midbodies in dividing HeLa cells, and the knockdown of CHMP1b reduces the amount of spastin at midbodies.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-CHMP1B, Cat. No. ABE1974, is a highly specific rabbit polyclonal antibody that targets charged multivesicular body protein 1b and has been tested in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected CHMP1B in Western Blotting applications (Morita, E., et. al. (2010). Proc Natl Acad. Sci USA. 107(29):12889-94; Bailey, J.K., et. al. (2015). J Biol Chem. 290(14):8987-9001).
Biological Information
ImmunogenGST-tagged full length recombinant human Charged multivesicular body protein 1b.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects Charged multivesicular body protein 1b in human cells. Human CHMP1B displays 99% and 98% homology with bovine and mouse CHMP1B, respectively.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react bovine and mouse based on 99 and 98% homology, respectively.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CHMP1B
  • C18orf2
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~29 kDa observed; 22.11 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in ARPE-19 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected CHMP1B in 10 µg of ARPE-19 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1974 04054839201004