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ABE1307 Anti-CHD1L Antibody

Anti-CHD1L Antibody is an antibody against CHD1L for use in Western Blotting.

Anti-CHD1L Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: CHD1L, ALC1 UniProt Number: Q86WJ1
ABE1307
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1307
DescriptionAnti-CHD1L Antibody
Alternate Names
  • Chromodomain-helicase-DNA-binding protein 1-like
  • Amplified in liver cancer protein 1
  • CHD1L
Background InformationChromodomain helicase/ATPase DNA binding protein 1-like gene (CHD1L) is a ~100.9 kDa DNA helicase that is amplified in many solid tumors. The CHD1L gene, located at Chr 1q21 is frequently amplified in hepatocellular carcinoma. CHD1L plays a role in chromatin remodeling following DNA damage. It is targeted to DNA damage sites through interaction with poly(ADP)-ribose) and regulated chromatin during DNA repair. CHD1L contains a SNF2-N domain and a helicase superfamily domain, but unlike CHD1, it does not contain a chromodomain, but instead contains a c-terminal macrodomain, which is an (ADP)-ribose/ polymer of the ADP-ribose (PAR)-binding element. In addition to PAR binding, the macro domain of CHD1L is able to bind Nur77, a critical member of a p53-independent apoptotic pathway. This binding subsequently inhibits the nucleus-to-mitochondria translocation of Nur77 and disrupts apoptotic cell death. Overexpression of CHD1L protein in tumors is considered to be an independent biomarker of poor prognosis and short tumor-free survival time.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-CHD1L Antibody is an antibody against CHD1L for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected CHD1L in 10 µg of Jurkat extract.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the Helicase C-terminal domain of human CHD1L.
EpitopeHelicase C-terminal domain
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis isoform has been observed to react with isoform 1 and 3.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse, Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CHD1L
  • ALC1
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~78/110 kDa observed. Uncharacterized band(s) may appear in some lysates. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CHD1L in 10 µg of A549 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1307 04055977147186