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CBL555 Anti-CD34 Class III Antibody, clone 581

CBL555
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCMPurifiedMonoclonal Antibody
Description
Catalogue NumberCBL555
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-CD34 Class III Antibody, clone 581
Product Information
FormatPurified
PresentationProtein G affinity chromatography. The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Quality LevelMQ100
Applications
ApplicationThis Anti-CD34 Antibody, Class III, clone 581 is validated for use in FC for the detection of CD34 Class III.
Key Applications
  • Flow Cytometry
Application NotesStudy of the Class III CD34 epitope by FACS

Optimal working dilutions must be determined by the end user.
Biological Information
Clone581
HostMouse
SpecificityClone 581 reacts with the class III CD34 epitope is resistant to neuraminidase, chymopapain and glycoprotease.

The antibody reacts with a single-chain 105-120 kDa heavily O-glycosylated transmembrane glycoprotein which is expressed on haematopoietic progenitor cells, vascular endothelium and some tissue fibroblasts. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. CD34 may also play a role in the adhesion of specific antigens to endothelium.
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryCD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells.[supplied by OMIM]
Gene Symbol
  • CD34
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P28906 # Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
SIZE: 385 amino acids; 40716 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
DEVELOPMENTAL STAGE: On early hematopoietic progenitor cells.
PTM: Highly glycosylated. & Phosphorylated on serine residues by PKC.
DISEASE:SwissProt: P28906 # Abnormal CD34 expression in leukemogenesis.
SIMILARITY: SwissProt: P28906 ## Belongs to the CD34 family.
Product Usage Statements
Availability by Geography
  • This product is not available for sale in the United States.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
CBL555 04053252506802