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MABF1758 Anti-CD3 (human), redFluor™ 710, clone UCHT1 Antibody

This mouse monoclonal Anti-CD3 Antibody (human), redFluor™ 710, clone UCHT1, Cat. No. MABF1758 is validated for use in Flow Cytometry, for the detection of CD3.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: CD3E, T3E UniProt Number: P07766
MABF1758
25 tests  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCMredFluor™ 710Monoclonal Antibody
Description
Catalogue NumberMABF1758
DescriptionAnti-CD3 (human), redFluor™ 710, clone UCHT1 Antibody
Alternate Names
  • T-cell surface glycoprotein CD3 epsilon chain
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • CD antigen CD3e
  • CD3
Background InformationThe UCHT1 antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines or activationinduced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The UCHT1 antibody is a widely used phenotypic marker for human T cells. In addition, binding/cross-linking of UCHT1 antibody to CD3e can induce cell activation. A recent publication of the crystal structure of a CD3e- antibody complex provides insight as to the action of commonly used agonist antibodies, as well as specific epitope-binding data for the human CD3 antibodies UCHT1 and OKT3 (Fernandes, R.A. et al. 2012. J. Biol. Chem. 287: 13324-13335).UCHT1 antibody reacts with both surface-expressed and intracellular CD3e protein, in contrast to an alternative human CD3 clone, HIT3a, which will stain only the extracellular (membrane-expressed) CD3e protein.
References
Product Information
FormatredFluor™ 710
PresentationPurified Mouse monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Quality LevelMQ100
Applications
ApplicationThis mouse monoclonal Anti-CD3 Antibody (human), redFluor™ 710, clone UCHT1, Cat. No. MABF1758 is validated for use in Flow Cytometry, for the detection of CD3.
Key Applications
  • Flow Cytometry
Biological Information
ImmunogenImmunogen information for this antibody is proprietary information.
CloneUCHT1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • CD3E
  • T3E
Purification MethodPurified by size-exclusion chromatography
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceThis monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 μg from a representative lot detected CD3 in one million human peripheral blood lymphocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Packaging Information
Material Size25 tests
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF1758 04055977290530

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies