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MABF397 Anti-CD25 Antibody (mouse), PE-Cy7, clone PC61.5

This rat monoclonal antibody Anti-CD25 (mouse), PE-Cy7, clone PC61.5 validated for use in IP, IHC & Flow Cytometry for the detection of CD25.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: Il2ra, Il2r UniProt Number: P01590
MABF397
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MIP, IHC, FCRPE-Cy7Monoclonal Antibody
Description
Catalogue NumberMABF397
DescriptionAnti-CD25 Antibody (mouse), PE-Cy7, clone PC61.5
Alternate Names
  • Interleukin-2 receptor subunit alpha
  • IL-2 receptor subunit alpha
  • IL-2-RA
  • IL-2R subunit alpha
  • IL2-RA
  • p55
  • CD25
Background InformationThe PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, althoµgh with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells.The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used for depletion of Treg cells in vivo, as well as to distinguish Treg cells from naïve or conventional T cells which are CD25-.
Product Information
FormatPE-Cy7
PresentationPurified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis rat monoclonal antibody Anti-CD25 (mouse), PE-Cy7, clone PC61.5 validated for use in IP, IHC & Flow Cytometry for the detection of CD25.
Key Applications
  • Immunoprecipitation
  • Immunohistochemistry
  • Flow Cytometry
Biological Information
ImmunogenImmunogen information for this antibody is proprietary information.
ClonePC61.5
Concentration0.2 mg/mL
HostRat
IsotypeIgG1λ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Gene Symbol
  • Il2ra
  • Il2r
Purification MethodSize Exclusion Chromatography
UniProt Number
Product Usage Statements
Quality AssuranceThe monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 µg of this antibody detected CD25 in one million C57Bl/6 splenocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsIt is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
MABF397 04053252959899