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480716 α2-3,6,8,9-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: N-Acetylneuraminyl Hydrolase, Sialidase

480716
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Overview

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Catalogue NumberPackaging Qty/Pack
480716-250MIU Plastic ampoule 250 miu
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Description
OverviewRecombinant, Arthrobacter ureafaciens α2-3,6,8,9-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. High concentrations of this sialidase and prolonged periods of incubation result in the additional cleavage of branched sialic acids. This enzyme is found as two active species of MW 60000 and 69000.
Note: 1 mU = 1 milliunit.
Catalogue Number480716
Brand Family Calbiochem®
SynonymsN-Acetylneuraminyl Hydrolase, Sialidase
References
ReferencesMeans, R.E. and Desrosiers, R.C. 2000. J. Virol. 74, 11181.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Roggentin, P., et al. 1995. Biol. Chem. Hoppe Seyler 376, 569
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Product Information
CAS number9001-67-6
Activity≥5 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid per min at 37°C, pH 5.0. (Note: 1 mU = 1 milliunit).
EC number3.2.1.18
FormLiquid
FormulationIn 20 mM Tris-HCl, 20 mM NaCl, pH 7.5.
Quality LevelMQ100
Biological Information
Specific Activity≥40 units/mg protein
Physicochemical Information
ContaminantsN-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases: none detected.
Safety Information according to GHS
RTECSQQ3450000
Safety Information
R PhraseR: 20-36/37/38-42/43

Harmful by inhalation.
Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Harmful
Storage +2°C to +8°C
Do not freeze Yes
Global Trade Item Number
Catalogue Number GTIN
480716-250MIU 04055977201437