Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Digitonin, High Purity- is a nonionic detergent commonly used to solubilize membrane-bound proteins. Digitonin is an ideal tool for membrane protein research, and has proven to be especially useful in the study of neurotransmitter receptors.
OmniPur® Sodium Dodecyl Sulfate- can be used to lyse cells during DNA extraction and to unfold proteins for SDS-PAGE. SDS denatures proteins by disrupting non-covalent bonds that maintain native conformation.
Below are highlights and summaries from the Essential Biochemicals for Research, which is a technical resource and product guide for scientists for the preparation and use of biochemicals such as antibiotics, buffers, detergents, dyes, stains, and substrates, which are indispensable for any life science research laboratory. To view the full content, please download or request a print copy of this resource.
Common Properties of Detergents
Critical Micelle Concentration (CMC) The CMC can be defined as the lowest concentration above which monomers cluster to form micelles.
Kraft Point The temperature at which all the three phases— crystalline, micellar, and monomeric—exist in equilibrium is known as as the Kraft Point.
Cloud Point At a particular temperature above the CMT, nonionic detergents become cloudy and undergo phase separation to yield a detergent-rich layer and an aqueous layer.
Protocols for Using Detergents
Removal of Unbound Detergents Excess detergent is normally used to solubilize membrane proteins. This is to ensure complete dissolution of the membrane and to provide a large number of micelles such that only one protein molecule is present per micelle. Some of the more commonly used methods for detergent removal are:
Hydrophobic Adsorption
Gel Chromatography
Centrifugal Ultrafiltration
Dialysis
Ion-Exchange Chromatography
Guidelines for Choosing a Detergent In most cases, the biological activity of the protein should be preserved in the supernatant after detergent solubilization. Hence, the appropriate detergent should yield the maximum amount of biologically active protein in the supernatant. Some of the points outlined below can be helpful in selecting a suitable detergent.
Survey the literature
Consider the solubility of the detergent at the working temperature