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70003 pBAC™-1 DNA - Novagen

70003
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
70003-3 Plastic ampoule 10 μg
Description
OverviewpBAC™-1 is a baculovirus transfer plasmid designed for simplified cloning and expression of target genes in insect cells. The plasmid is compatible with BacVector™-1000, -2000 or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the polh promoter. Inserts in pBAC-1 must contain their own translation initiation codon. The vector encodes an optional C-terminal His•Tag® fusion sequence that may be utilized if the insert allows read-through in the proper reading frame. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Single stranded sequencing of phage-derived DNA can be performed using the 1629DWN primer.





This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
Catalogue Number70003
Brand Family Novagen®
References
Product Information
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
70003-3 04055977273830

Documentation

pBAC™-1 DNA - Novagen SDS

Title

Safety Data Sheet (SDS) 

pBAC™-1 DNA - Novagen Certificates of Analysis

TitleLot Number
70003

Brochure

Title
Insect Cell Expression

Citations

Title
  • Zhongmin Ma, et al. (1999) Human pancreatic islets express RNA species encoding two distinct catalytically active isoforms of group VI phospholipase A2 (iPLA2) that arise from an exon-skipping mechanism of alternative splicing of the transcript from the iPLA2 gene on the chromosome. Journal of Biological Chemistry 274, 9607-9616.
  • Antonio Alcami, et al. (1998) Blockade of chemokine activity by a soluble chemokine binding protein from vaccinia virus. Journal of Immunology 160, 624-633.
  • Robert Bals, et al. (1998) Human defensin 2 Is a salt-sensitive peptide antibiotic expressed in human lung. Journal of Clinical Investigation 102, 874-880.
  • Vector Map

    Title
    TB127 pBAC-1 Vector Map

    Vector Sequence

    Title
    pBAC-1 Sequence