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509887 miR-372/3 Inhibitor, Neomycin-S - Calbiochem

A cell-permeable neomycin derived artificial nucleobase conjugate. Inhibits the production of oncogenic miRNAs 372 (IC₅₀ = 2.4 µM) by binding to pre-miRNAs and interrupts processing by Dicer.

miR-372/3 Inhibitor, Neomycin-S - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: miRNA-372 Inhibitor, miRNA-373 Inhibitor

Primary Target: miRNA-272/273
509887
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Overview

Replacement Information

Key Spec Table

Empirical Formula
C₃₉H₆₀O₁₄N₁₂S

Products

Catalogue NumberPackaging Qty/Pack
5098870001 Glass bottle 2 mg
Description
OverviewA cell-permeable neomycin derived artificial nucleobase conjugate that inhibits the production of oncogenic miRNAs 372 (IC50 = 2.4 µM; Kd = 16 nM) and 373 (IC50 = 5.13 µM Kd = 21.4 nM) by directly binding to their precursors pre-miRNAs and interrupts their processing by Dicer. In comparison, neomycin does not affect these miRNAs (IC50 = 125 µM; Kd = 7.4 µM). Shown to depress proliferation of AGS gastric cancer cells expressing miRNA 372 and 373. However, it has no effect on MKN74 gastric epithelial cells that do not express miRNA 372 and 373. Reduced levels of miRNA 372 results in de-repression of their targets, such as the cell cycle regulator large tumor suppressor homologue 2 (LATS2).

Please note that the molecular weight for this compound is batch-specific due to variable water content.
Catalogue Number509887
Brand Family Calbiochem®
SynonymsmiRNA-372 Inhibitor, miRNA-373 Inhibitor
References
ReferencesVo, D.D., et al. 2014. ACS Chem. Biol. 9, 711.
Product Information
FormOff-white solid
Hill FormulaC₃₉H₆₀O₁₄N₁₂S
Chemical formulaC₃₉H₆₀O₁₄N₁₂S
Hygroscopic Hygroscopic
ReversibleY
Quality LevelMQ100
Applications
Biological Information
Primary TargetmiRNA-272/273
Purity≥98% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage ≤ -70°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-2-°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
5098870001 04055977241150

Documentation

miR-372/3 Inhibitor, Neomycin-S - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Vo, D.D., et al. 2014. ACS Chem. Biol. 9, 711.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-June-2014 JSW
SynonymsmiRNA-372 Inhibitor, miRNA-373 Inhibitor
DescriptionA cell-permeable neomycin derived artificial nucleobase conjugate that inhibits the production of oncogenic miRNAs 372 (IC50 = 2.4 µM; Kd = 16 nM) and 373 (IC50 = 5.13 µM Kd = 21.4 nM) by directly binding to their precursors pre-miRNAs and interrupts their processing by Dicer. In comparison, neomycin does not affect these miRNAs (IC50 = 125 µM; Kd = 7.4 µM). Shown to depress proliferation of AGS gastric cancer cells expressing miRNA 372 and 373. However, it has no effect on MKN74 gastric epithelial cells that do not express miRNA 372 and 373. Reduced levels of miRNA 372 results in de-repression of their targets, such as the cell cycle regulator large tumor suppressor homologue 2 (LATS2).
FormOff-white solid
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₃₉H₆₀O₁₄N₁₂S
Purity≥98% by HPLC
SolubilityDMSO (100 mg/ml)
Storage Protect from light
≤ -70°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-2-°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesVo, D.D., et al. 2014. ACS Chem. Biol. 9, 711.