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69522 T7•Tag® Monoclonal Antibody

The T7•Tag antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors.

T7•Tag® Monoclonal Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
69522
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
69522-3 Plastic ampoule 50 μg
69522-4 Plastic ampoule 250 μg
Description
OverviewThe T7•Tag® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN™ and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select™ Vectors). For increased versatility and sensitivity, this antibody is also available labeled with biotin and as a direct conjugate with alkaline phosphatase or horseradish peroxidase.
SpecificityT7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal
Species/IsotypeMouse monoclonal IgG2b
Cross-reactivityNegligible with bacterial, insect or mammalian cell lysates
Sensitivity1–10 ng (Western blot developed with chromogenic substrates)
< 1 ng (AP or HRP conjugate developed with chemiluminescent substrates, or Biotin conjugate developed with Streptavidin AP and chromogenic substrates). Also suitable for immunoprecipitation and immunohistochemistry (1–3)
FormStabilized solution of antibody in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein
Working dilution1:5,000–1:10,000
Catalogue Number69522
Brand Family Novagen®
References
References1. Krek, W., et al. 1994. Cell 78,, 161.

2. Roberts, S.G.E. and Green, M. (1994) Nature 371, 717–720.

3. Joneson, T., McDonough, M., Bar-Sag, D., and Van Aelst, L. (1996) Science 274, 1374–1376.
Product Information
Components
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Multiple Toxicity Values, refer to MSDS
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
69522-3 04055977274448
69522-4 07790788052584

Documentation

T7•Tag® Monoclonal Antibody SDS

Title

Safety Data Sheet (SDS) 

T7•Tag® Monoclonal Antibody Certificates of Analysis

TitleLot Number
69522

References

Reference overview
1. Krek, W., et al. 1994. Cell 78,, 161.

2. Roberts, S.G.E. and Green, M. (1994) Nature 371, 717–720.

3. Joneson, T., McDonough, M., Bar-Sag, D., and Van Aelst, L. (1996) Science 274, 1374–1376.

Citations

Title
  • Clock, S.A., et al. 2008. Journal of Bacteriology 190, 980.
  • Chunying Song, et al. 2008. Journal of Biological Chemistry 283, 4004.
  • Cinar, B., et al. 2007. Journal of Biological Chemistry 282, 29584.
  • Cocuron, J., et al. 2007. Procedings of the National Academy of Science 104, 8550.
  • Furuyama, S. and Biggins S., 2007. Procedings of the National Academy of Science 104, 14706.
  • Geisler, D.A, et al. 2007. Journal of Biological Chemistry 282, 28455.
  • Goodier, J.L., et al. 2007. Molecular and Cellular Biology 27, 6469.
  • Hayakawa, H., et al. 2007. "http://www.jbc.org/content/282/36/26369.abstract">Journal of Biological Chemistry 282, 26369.
  • Kennedy, N.J., et al. 2007. Genes and Development 21, 2336.
  • Maddugoda, M.P., et al. 2007. Journal of Cell Biology 178, 529.
  • Schoonheim, P.J., et al. 2007. Plant Physiology 143, 670.
  • Ye, Z., et al. 2007. American Journal of Pathology 171, 882.
  • Bedet, C., et al. 2006. Journal of Biological Chemistry 281, 30046.
  • Bor, Y., et al. 2006. Genes and Development 20, 1597.
  • Chia, D.J., et al. 2006. Journal of Biological Chemistry 281, 3190.
  • Dance, M., et al. 2006. Journal of Biological Chemistry 281, 23285.
  • Johnson, E.A., et al. 2006. Molecular Pharmacology 70, 676.
  • Leger, A.J., et al. 2006. Circulation 113, 1244.
  • Nowak, J.E., et al. 2006. Bio/Techniques 40, 220.
  • Oh, R.S., et al. 2006. Journal of Biological Chemistry 281, 21480.
  • Rayala, S.K., et al. (2006. Journal of Biological Chemistry 281, 4395.
  • Taira, H., et al. 2006. Nucleic Acids Research 34, 1653.
  • Uchimura, Y., et al. 2006. Journal of Biological Chemistry 281, 23180-23190.
  • Zhang, F., et al. 2006. Journal of Biological Chemistry 281, 30036.
  • Allemand, E., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 3605.
  • Bairstow, S.F., et al. 2005. Journal of Biological Chemistry 280, 23884.
  • Barboric, M., et al. 2005. European Molecular Biology Organization Journal 24, 4291.
  • Barnes, C.J., et al. 2005. Clinical Cancer Research 11, 2868.
  • Chisholm, S.T., et al. 2005. Procedings of the National Academy of Science 102, 2087.
  • Chuang C., and Bargmann, C.I., 2005. Genes and Development 19, 270.
  • Cui, H., et al. 2005. Journal of Biological Chemistry 280, 9474.
  • Darnell, J.C., et al. 2005. Genes and Development 19, 903.
  • Dasgupta, B., et al. 2005. Cancer Research 65, 2755.
  • Dasgupta, B. et al. 2005. Cancer Research 65, 236.
  • Dredge, B.K., et al. 2005. European Molecular Biology Organization Journal 24, 1608.
  • Georgescu, S.P., et al. 2005. Molecular Endocrinology 19, 2491.
  • Huang, A., et al. 2005. Cancer Research 65, 5607.
  • Katagiri, C., et al. 2005. Journal of Biological Chemistry 280, 14716.
  • Kotoshiba, S., et al. 2005. Journal of Biological Chemistry 280, 17694.
  • Kray, A.E., et al. 2005. Journal of Biological Chemistry 280, 35974.
  • Kuroda T.S., and Fukuda, M., 2005. Journal of Biological Chemistry 280, 28015.
  • Lin, Y., et al. 2005. Journal of Biological Chemistry 280, 12799.
  • Lucet, I.S., et al. 2005. Journal of Biological Chemistry 280, 2503.
  • Miyake, Y., et al. 2005. Journal of Biological Chemistry 280, 12643.
  • Miyase, S., et al. 2005. Journal of Biological Chemistry 280, 515.
  • Nakamura, N., et al. 2005. Journal of Biological Chemistry 280, 1561.
  • Papandreou, I., et al. 2005. Cancer Research 65, 3171.
  • Qiu, R., et al. 2005. Journal of Biological Chemistry 280, 16695.
  • Robitaille, H., et al. 2005. Journal of Biological Chemistry 280, 12732.
  • Sun, L., et al. 2005. Journal of Biological Chemistry 280, 26483.
  • Suzuki, K., et al. 2005. Biophysical Journal 88, 3659.
  • Thomee, C., et al. 2005. Journal of Clinical Endocrinology and Metabolism 90, 2487.
  • Vo, A.T., et al. 2005. European Molecular Biology Organization Reports 6, 438.
  • Wente, W., et al. 2005. Journal of Biological Chemistry 280, 32419.
  • Wheeler, D.L., et al. 2005. American Journal of Pathology 166, 117.
  • Yanagi, K., et al. 2005. Journal of Biological Chemistry 280, 19689.
  • Zhao X., and Blobel G., 2005. Proceedings of the National Academy of Sciences (USA) 102, 4777.
  • Ball, J.R., et al. 2004. RNA 10, 19.
  • Fukuda, M. and Kuroda T.S., 2004. Journal of Cell Science 117, 583.
  • Kurebayashi, S., et al. 2004. Biochemical and Biophysical Research Communications 315, 919.
  • Tan, W., et al. 2004. Journal of Biological Chemistry 279, 55153.
  • Cobbold, C., et al. 2003. Human Molecular Genetics 12, 1523.
  • Kagey, M.H., et al. 2003. Cell 113, 127.
  • Kim E.J., and Helfman, D.M., 2003. Journal of Biological Chemistry 278, 30063.
  • Han, Z., et al. 2002. Biochemical and Biophysical Research Communications 292, 1036.
  • Zhong, H., et al. 2002. Nature 420, 193.
  • Sekiguchi, T., et al. 2001. Journal of Biological Chemistry 276, 7246.
  • Morimura, T., et al. 2000. Journal of Biological Chemistry 275, 36523.

    • User Protocols

    Title
    TB015 T7•Tag® Antibody