569393 Sigma-AldrichStauprimide - CAS 154589-96-5 - Calbiochem
A cell-permeable ESCs (Embryonic Stem Cells) EMT (Epithelial-Mesenchymal Transition) inducer that is reported to prime/sensitize human and murine ESC cultures for much more enhanced/efficient differen
More>> A cell-permeable ESCs (Embryonic Stem Cells) EMT (Epithelial-Mesenchymal Transition) inducer that is reported to prime/sensitize human and murine ESC cultures for much more enhanced/efficient differentiation into progenitor cells of multiple lineages. Less<<Stauprimide - CAS 154589-96-5 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Synonyms: Spd, ESC EMT Inducer
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Overview
Key Spec Table
| CAS # | Empirical Formula |
|---|---|
| 154589-96-5 | C₃₅H₂₈N₄O₅ |
Products
| Catalogue Number | Packaging | Qty/Pack | |
|---|---|---|---|
| 569393-500UG | 500 μg |
| Description | |
|---|---|
| Overview | A cell-permeable ESCs (Embryonic Stem Cells) EMT (Epithelial-Mesenchymal Transition) inducer that is reported to prime/sensitize human and murine ESC cultures for much more enhanced/efficient differentiation into progenitor cells of multiple lineages, including Activin A- (Cat. No. 114700) induced definitive endoderm differentiation (60% differentiation from murine R1 ESCs with 5 ng/ml Activin A and 200 nM Spd), ATRA- (Cat. No. 554720) induced neuronal progenitor differentiation, and BMP-4- (Cat. No. 203642) induced mesoderm differentiation, under suboptimal lineage specifying stimuli concentrations and often serum-free conditions, while Spd sensitization alone does not induce any ESCs differentiation. Spd is shown to interact with NME2/DNPK B/PUF and prevent NME2 nuclear localization and c-myc transcription in murine R1 ESC cultures (46% and 85% drop in c-myc mRNA in 24 h, respectively, with 200 nM and 500 nM Spd), which accounts for, at least in part, its EMT induction activity. Although a structural analog of the broad-spectrum kinase inhibitors Staurosporine (Cat. Nos. 569396 and 569397) and UCN-01 (Cat. No. 539644), Spd exhibits significant kinase inhibitory activities only at elevated concentrations, but not at concentrations ≤500 nM typically used in EMT induction. |
| Catalogue Number | 569393 |
| Brand Family | Calbiochem® |
| Synonyms | Spd, ESC EMT Inducer |
| References | |
|---|---|
| References | Zhu, S., et al. 2009. Cell Stem Cell 4, 416. |
| Product Information | |
|---|---|
| CAS number | 154589-96-5 |
| Form | Yellow solid |
| Hill Formula | C₃₅H₂₈N₄O₅ |
| Chemical formula | C₃₅H₂₈N₄O₅ |
| Structure formula Image | |
| Quality Level | MQ100 |
| Biological Information | |
|---|---|
| Purity | ≥95% by HPLC |
| Safety Information | |
|---|---|
| S Phrase | S: 22-24/25 Do not breathe dust. Avoid contact with skin and eyes. |
| Packaging Information | |
|---|---|
| Packaged under inert gas | Packaged under inert gas |
| Global Trade Item Number | |
|---|---|
| Catalogue Number | GTIN |
| 569393-500UG | 04055977190441 |