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SAE0109-100UG SAvPhire Monomeric Streptavidin alkaline phosphatase conjugate

SAvPhire Monomeric Streptavidin,Cat.#SAE0109, is a high-affinity recombinant variant of SA that minimizes the risk of aggregation, precipitation, and crosslinking in pre-incubation with biotinylated molecules and offers easier access to the desired targets, tested for use in IHC (Paraffin).

SAE0109-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSAE0109-100UG
DescriptionSAvPhire Monomeric Streptavidin alkaline phosphatase conjugate
Alternate Names
  • SAVphire-AP
  • mSA-AP
Background InformationStreptavidin, a tetrametic protein, has been extensively used in purification and detection of biomolecules. The strong streptavidin-biotin bond can highly suited for attaching various biomolecules to one another or onto a solid support. The streptavidin-biotin complex displays high resistance to organic solvents, denaturants, detergents, and proteolytic enzymes. It can also withstand extremes of temperature and pH. However, one challenge faced by researchers using the streptavidin-biotin system is the target aggregation, which results from multivalent binding. It can cause cross-linking that can interfere with observations at the molecular level. These challenges can be overcome by use of monomeric streptavidin (mSA) that displays structural and binding properties that are suitable for multiple applications based on its small size and monovalent biotin binding. The monomeric form allows for association with biotinylated ligands without inducing aggregation that are observed with multivalent interactions. Monomeric form can also be genetically fused to another protein to form a biotin-binding tag. SAvPhire Monomeric Streptavidin is a is a high-affinity recombinant variant of streptavidin that minimizes the risk of aggregation, precipitation, and crosslinking in pre-incubation steps with biotinylated molecules and offers easier access to the desired targets. Unlike other non-engineered monomeric forms, SAvPhire Monomeric Streptavidin displays highly desirable dissociation kinetics (koff = 63 x 10-3 min-1 for non-engineered monomeric form and 2.1 x 10-3 min-1 for SAvPhire Monomeric Streptavidin) and longer t1/2 (11 min vs 329 min). It is suitable for multiple immunodetection applications. (Ref.: Kroestch, A., et al., (2018). Appl. Microbiol. Biotechnol., 102(23); 10079-10089).
References
Product Information
FormatAlkaline Phosphatase
PresentationMonomeric Streptavidin in buffer containing 0.05 M Tris, 0.15 M NaCl, 1 mM MgCl2, 0.2% BSA, pH 8.0 with 0.1% sodium azide with 10% glycerol.
Applications
ApplicationSAvPhire Monomeric Streptavidin,Cat.#SAE0109, is a high-affinity recombinant variant of SA that minimizes the risk of aggregation, precipitation, and crosslinking in pre-incubation with biotinylated molecules and offers easier access to the desired targets, tested for use in IHC (Paraffin).
Key Applications
  • Immunohistochemistry (Paraffin)
Application NotesEvaluated by Immunohistochemistry (Paraffin) with Anti-NeuN antibody (ABN78) for detection of NeuN in mouse brain tissue sections.

Immunohistochemistry (Paraffin) Analysis (IHC(P)): A 1:500 dilution Anti-NeuN (ABN78) was used to stain sections of mouse cerebral cortex and signal was detected with Savphire monomeric streptavidin alkaline phosphatase conjugate (1:500 dilution) and Vulcan Fast Red.
Biological Information
ImmunogenN/A
ConcentrationPlease refer to lot specific datasheet.
HostE. coli
SpecificitySAvPhire Monomeric Streptavidin expressed in E. coli and conjugated with alkaline phosphatase is suitable for immunodetection applications. SAvPhire Monomeric Streptavidin is expressed with an N-terminal His-tag and a C-terminal FLAG® tag.
Species Reactivity
  • All
Species Reactivity NotePredicted to react with all species.
Antibody TypeMonoclonal Antibody
UniProt Number
Molecular Weight15.5 kDa for Savphire monomeric streptavidin (with an N-terminal His-tag and a C-terminal FLAG® tag).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) with Anti-NeuN antibody (ABN78) for detection of NeuN in mouse brain tissue sections.

Immunohistochemistry (Paraffin) Analysis (IHC(P)): A 1:500 dilution Anti-NeuN (ABN78) was used to stain sections of mouse cerebral cortex and signal was detected with Savphire monomeric streptavidin alkaline phosphatase conjugate (1:500 dilution) and Vulcan Fast Red.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SAE0109-100UG 04061841276777

Documentation

SAvPhire Monomeric Streptavidin alkaline phosphatase conjugate SDS

Title

Safety Data Sheet (SDS) 

SAvPhire Monomeric Streptavidin alkaline phosphatase conjugate Certificates of Analysis

TitleLot Number
SAvPhire Monomeric Streptavidin alkaline phosphatase conjugate - 3813903 3813903
SAvPhire Monomeric Streptavidin alkaline phosphatase conjugate - 4093859 4093859
SAvPhire Monomeric Streptavidin alkaline phosphatase conjugate - 4198581 4198581
SAvPhire Monomeric Streptavidin alkaline phosphatase conjugate - Q3327736 Q3327736