71400 Sigma-AldrichRosetta™ 2(DE3) Singles™ Competent Cells - Novagen
Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.
More>> Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. Less<<MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Recommended Products
Overview
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Products
| Catalogue Number | Packaging | Qty/Pack | |
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| 71400-3 | Plastic ampoule | 11 rxn | |
| 71400-4 | Glass bottle | 22 rxn |
| Description | |
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| Overview | A common method for transformation of DNA plasmids into E. coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen prepared competent cells. Novagen® Singles™ Competent Cells are designed for ultimate convenience and reliability in plasmid transformation. Chemically competent cells are prepared using an optimized method. Cells are provided in 50µl volumes, eliminating the need to aliquot, freeze/thaw, or waste partially used vials, saving time and money and ensuring reliable cell performance. To use, simply thaw, add DNA, incubate 5 minutes on ice, heat shock for 30 seconds, place on ice for 2 minutes, and add SOC medium.
T7 expression strains are lysogens of bacteriophage
DE3, as indicated by the (DE3). These hosts carry a
chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in appropriate T7 expression vectors, using IPTG as an inducer. Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. |
| Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE2 (CamR)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com. |
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| Catalogue Number | 71400 |
| Brand Family | Novagen® |
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| Product Information | ||
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| • | 11 rxn or 22 rxn | Rosetta 2(DE3) Competent Cells |
| • | 2 × 2 ml or 4 × 2 ml | SOC Medium |
| • | 10 µl | Test Plasmid |
| Guaranteed efficiency | >2 × 10⁶ cfu/µg | |
| Quality Level | MQ100 | |
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| Storage and Shipping Information | |
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| Ship Code | Dry Ice Only |
| Toxicity | Multiple Toxicity Values, refer to MSDS |
| Storage | ≤ -70°C |
| Do not freeze | Ok to freeze |
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| Specifications |
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| Global Trade Item Number | |
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| Catalogue Number | GTIN |
| 71400-3 | 04055977269963 |
| 71400-4 | 04055977269970 |
Documentation
Rosetta™ 2(DE3) Singles™ Competent Cells - Novagen SDS
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Rosetta™ 2(DE3) Singles™ Competent Cells - Novagen Certificates of Analysis
| Title | Lot Number |
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| 71400 |
Brochure
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| The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression |
Citations
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User Protocols
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| TB009 Competent Cells |
| TB053 Academic and Non-profit Laboratory Assurance Letter |
| TB055 pET System Manual |