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HTS058RTA Ready-to-Assay™ MRGPRX2 Mas-Related Gene Receptor Frozen Cells

Human MRGX2 / MRGPRX2 GPCR frozen cells for Calcium Flux FLIPR Assays.

Host Cell: Chem-1 Entrez Gene Number: NM_054030
HTS058RTA
1 kit  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

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This product requires a User Agreement (Label License). Please see product datasheet for further information

Key Spec Table

SpeciesHost CellsProtein Target
Human Chem-1MRGX2 / MRGPRX2
Description
Catalogue NumberHTS058RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ MRGPRX2 Mas-Related Gene Receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

MRGPRX2 (MrGX2) is a Gq- and Gi-coupled GPCR that binds to several peptides, including the neuropeptide cortistatin-14 and the proadrenomedullin N-terminal peptide (PAMP). Dorsal root ganglia and adrenal chromaffin cells express MRGPRX2, and binding of these ligands to MrgX2 is thought to play a role in nociception and catecholamine release from the adrenal glands (Robas et al., 2003; Kamohara et al., 2005). Millipore’s cloned human MRGPRX2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant MRGPRX2 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at MRGPRX2.
References
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
  • Fifty (50) mL of Media Component.
Detection methodFluorescent
Quality LevelMQ100
Applications
ApplicationHuman MRGX2 / MRGPRX2 GPCR frozen cells for Calcium Flux FLIPR Assays.
Key Applications
  • Calcium Flux Assays
Biological Information
Entrez Gene Number
GPCR ClassA
Host CellsChem-1
Protein TargetMRGX2 / MRGPRX2
Target Sub-FamilyMas-related gene
Cell Line Type
  • GPCR Cell Lines
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HTS058RTA 04053252377921

Documentation

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Categories

Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells