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HTS164RTA Ready-to-Assay™ GLP-2 Glucagon Family Receptor Frozen Cells

Human GLP-2 GPCR frozen cells for Calcium Flux FLIPR Assays.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-11 Entrez Gene Number: NM_004246
HTS164RTA
1 kit  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

SpeciesHost CellsProtein Target
Human Chem-11GLP-2
Description
Catalogue NumberHTS164RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ GLP-2 Glucagon Family Receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Glucagon-like peptide 2 (GLP-2) is a 33 amino acid peptide secreted by intestinal endocrine L cells in response to nutrient uptake. GLP-2 exerts multiple effects on intestinal physiology, including promotion of epithelial cell growth and survival to increase intestinal mass, increasing nutrient absorption capacity, and inhibition of intestinal inflammation (Dubé and Brubaker, 2007). The GLP-2 Receptor is a class B GPCR belonging to the glucagon/secretin family of receptors, and as with the other members of the family, activation of the GLP-2 receptor results in increased intracellular cAMP (Munroe et al., 1999). Millipore’s cloned human GLP-2 -expressing cell line is made in the Chem-11 host, which supports high levels of recombinant GLP-2 expression on the cell surface and contains optimized levels of a recombinant promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at GLP-2.
References
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
Detection methodFluorescent
Quality LevelMQ100
Applications
ApplicationHuman GLP-2 GPCR frozen cells for Calcium Flux FLIPR Assays.
Key Applications
  • Calcium Flux Assays
Biological Information
Entrez Gene Number
GPCR ClassB
Host CellsChem-11
Protein TargetGLP-2
Target Sub-FamilyGlucagon
Cell Line Type
  • GPCR Cell Lines
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HTS164RTA 04053252552625

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Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells