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506026 REST Inhibitor, X5050 - Calbiochem

A cell-permeable inhibitor repressor element-1 silencing transcription factor (REST). Targets REST degradation, but not its expression or binding to repressor element binding sequence.

REST Inhibitor, X5050 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Neuron Resctrictive Silencer Factor (NRSF) Inhibitor, X5050, X-5050, Repressor Element-1 Silencing Transcription Factor (REST) Inhibitor, X5050

Primary Target: REST
506026
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Overview

Replacement Information

Key Spec Table

Empirical Formula
C₁₇H₁₅N₃O₃

Products

Catalogue NumberPackaging Qty/Pack
5060260001 Glass bottle 25 mg
Description
OverviewA cell-permeable benzoimidazole derivative that acts as an inhibitor of repressor element-1 silencing transcription factor (REST, a Kruppel-type zinc finger transcription factor). Induces a dose-dependent reduction in the 122 kDa isoforms of REST in HEK cells. It is believed to target REST degradation, but not its expression or binding to repressor element binding sequence. Shown to rescue REST-regulated gene expressions, such as BDNF in the prefrontal cortex in mice with quinolinic acid-induced striatal lesions. Acute ventricular delivery of X5050 increases the expression of BDNFand other REST-regulated genes. Also shown to promote the expression of BDNF and SNAP25 in human neural stem cells via degradation of REST.

Please note that the molecular weight for this compound is batch-specific due to variable water content.
Catalogue Number506026
Brand Family Calbiochem®
SynonymsNeuron Resctrictive Silencer Factor (NRSF) Inhibitor, X5050, X-5050, Repressor Element-1 Silencing Transcription Factor (REST) Inhibitor, X5050
References
ReferencesCharbord, J., et al, 2013, Stem Cells 31, 1816.
Product Information
FormLight beige powder
Hill FormulaC₁₇H₁₅N₃O₃
Chemical formulaC₁₇H₁₅N₃O₃
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetREST
Purity≥97% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
5060260001 04055977262636

Documentation

REST Inhibitor, X5050 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

REST Inhibitor, X5050 - Calbiochem Certificates of Analysis

TitleLot Number
506026

References

Reference overview
Charbord, J., et al, 2013, Stem Cells 31, 1816.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-April-2016 JSW
SynonymsNeuron Resctrictive Silencer Factor (NRSF) Inhibitor, X5050, X-5050, Repressor Element-1 Silencing Transcription Factor (REST) Inhibitor, X5050
DescriptionA cell-permeable benzoimidazole derivative that acts as an inhibitor of repressor element-1 silencing transcription factor (REST, a Kruppel-type zinc finger transcription factor). Induces a dose-dependent reduction in the 122 kDa isoforms of REST in HEK cells. It is believed to target REST degradation, but not its expression or binding to repressor element binding sequence. Shown to rescue REST-regulated gene expressions, such as BDNF in the prefrontal cortex in mice with quinolinic acid-induced striatal lesions. Acute ventricular delivery of X5050 increases the expression of BDNFand other REST-regulated genes. Also shown to promote the expression of BDNF and SNAP25 in human neural stem cells via degradation of REST.
FormLight beige powder
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₇H₁₅N₃O₃
Structure formulaStructure formula
Purity≥97% by HPLC
SolubilityDMSO (100 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesCharbord, J., et al, 2013, Stem Cells 31, 1816.