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PS1013 PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb

This PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb is validated for use in Immunoblotting for the detection of VEGFR2 (pTyr1054/1059).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-KDR/Flk-1, Phospho-Specific (Tyr1054/1059), Anti-VEGF Receptor 2, Phospho-specific (pTyr1054/1059)

PS1013
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
MRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
PS1013-10T Plastic ampoule 10 t
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Description
OverviewRecognizes the ~200 kDa VEGFR-2 protein phosphorylated at Tyr1054/1059 in porcine pancreatic cells transfected with a CSF-1/mouse VEGFR-2 chimeric receptor.
Catalogue NumberPS1013
Brand Family Calbiochem®
SynonymsAnti-KDR/Flk-1, Phospho-Specific (Tyr1054/1059), Anti-VEGF Receptor 2, Phospho-specific (pTyr1054/1059)
Application Data
Detection of mouse VEGFR2, phosphorylated at Tyr1054/1059, by immunoblotting. Samples: Whole cell lysate from porcine aortic endothelial (PAE) cells transfected with CSF-1/mouse VEGFR2 chimeric receptor left unstimulated (lanes 1,2) or stimulated with CSF-1 (lanes 3-8). Primary antibodies: PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb (Cat. No. PS1013) (0.5 µg/ml) pre-incubated without peptide (lanes 2,4,5), with non-phosphopeptide corresponding to the immunogen (lane 6), a generic phosphotyrosine-containing peptide (lane 7), or the phosphopeptide immunogen (lane 8) or pan anti-VEGFR2 antibody (lanes 1,3). Detection: chemiluminescence.
References
ReferencesMeyer, R.D., et al. 2002. J. Biol. Chem. 277, 27081.
Zeng, H., et al. 2001. J. Biol. Chem. 276, 32714.
Sato, Y., et al. 2000. Ann. N.Y. Acad. Sci. 902, 201.
Rahimi, N., et al. 1999. Mol. Biol. Cell 10, 3401.
Product Information
FormLiquid
FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3 (± 0.1).
Positive controlPorcine aortic endothelial (PAE) cells transfected with a CSF-1/mouse VEGFR2 chimeric receptor
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:1000)
Application CommentsThe immunogen sequence is 100% conserved in human and rat VEGFR2, but reactivity has not been tested. The corresponding phosphorylation site in VEGFR3 is 100% conserved, but cross-reactivity has not been tested. When used at a 1:1000 dilution there is sufficient antibody for 10 immunoblots at 10 ml/blot. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1054/1059 phosphorylation sites of mouse and human VEGFR-2
HostRabbit
IsotypeIgG
Species Reactivity
  • Mouse
Antibody TypePolyclonal Antibody
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Irritant
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number GTIN
PS1013-10T 04055977227086