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33-040 PI 3-Kinase 3-step HTRF™ Assay

Everything you need to perform the PI3 Kinase 3-step HTRF assays (384 wells).

PI 3-Kinase 3-step HTRF™ Assay MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Kinase Assay
33-040
384 wells  Kit capacity: 384 wells
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Species ReactivityKey ApplicationsAnalytes AvailableDetection Methods
H, MKAPI 3-Kinase HTRF
Description
Catalogue Number33-040
Brand Family Upstate
Trade Name
  • HTRF
  • Upstate
DescriptionPI 3-Kinase 3-step HTRF™ Assay
Product Information
Components
  • 4X Reaction Buffer
  • PIP2 (substrate, 1mM stock)
  • DM A Reaction Buffer
  • DM B Reaction Buffer
  • DM C Reaction Buffer
  • BPIP3
  • Stop Reagent
Detection methodHTRF
Quality LevelMQ100
Applications
ApplicationEverything you need to perform the PI3 Kinase 3-step HTRF assays (384 wells).
Key Applications
  • Kinase Assay
Biological Information
Species Reactivity
  • Human
  • Mouse
Analytes Available
  • PI 3-Kinase
Entrez Gene Number
Entrez Gene SummaryPhosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
Gene Symbol
  • PIK3CA
  • MGC142163
  • PI3K
  • MGC142161
  • p110-alpha
Target Sub-FamilyPI3K/PI4K
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
SIZE: 1068 amino acids; 124284 Da
SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions12 months at -70°C
Packaging Information
Material Size384 wells
Material PackageKit capacity: 384 wells
Global Trade Item Number
Catalogue Number GTIN
33-040 04053252854378