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HCYTMAG-60K-PX30 MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel - Premixed 30 Plex - Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology Research Sub-Category: Inflammation & Autoimmune Mechanisms
HCYTMAG-60K-PX30
96-well plate  96 well plate
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsMultiplexing Ordering RequirementsDetection Methods
EGF G-CSF GM-CSF IFN-α2 IFN-γ IL-1α IL-1β IL-1ra IL-2 IL-3 IL-4 IL-5 IL-6 IL-7 IL-8 IL-10 IL-12 (p40) IL-12 (p70) IL-13 IL-15 IL-17A IP-10 MCP-1 MIP-1α MIP-1β TNF-α TNF-β VEGF RANTES Eotaxin/CCL11 HMplexNote: RANTES, PDGF-AA and PDGF-AB/AA cannot be plexed with other analytes for serum/plasmaLuminex xMAP
Description
Catalogue NumberHCYTMAG-60K-PX30
ReplacesHCYTA-60K-PX38
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel - Premixed 30 Plex - Immunology Multiplex Assay
OverviewTo identify specific cytokines involved in any inflammatory or immune response, it might be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing.

The MILLIPLEX® MAP Human Cytokine / Chemokine panel enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
Background Information“Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. Cytokines differ from hormones in that they act on a wider spectrum of target cells. Also, unlike hormones, they are not produced by specialized cells which are organized in specialized glands. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Cytokine and chemokine research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, as well as disease states such as inflammatory disease, allergic reactions, irritable bowel disease (IBD), sepsis, and cancer.
References
Product Information
Detection methodLuminex xMAP
HS Code3002 15 90
ConfigurationPremixed
Precision, %
  • Intra-assay: 4 - 13
  • Inter-assay: 5 - 17
Multiplex Ordering RequirementsNote: RANTES, PDGF-AA and PDGF-AB/AA cannot be plexed with other analytes for serum/plasma
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationSimultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended.

• This assay requires 25 µl of neat plasma or serum, or 25 μL cell culture supernatant per well.

• This kit may be used for the analysis of all or any combination of the cytokines and chemokines in serum, plasma or tissue/cell lysate and culture supernatant samples with the exception of RANTES. RANTES cannot be combined with all other cytokines in this panel in serum/plasma due to a required 1:100 dilution.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • EGF
  • G-CSF
  • GM-CSF
  • IFN-α2
  • IFN-γ
  • IL-1α
  • IL-1β
  • IL-1ra
  • IL-2
  • IL-3
  • IL-4
  • IL-5
  • IL-6
  • IL-7
  • IL-8
  • IL-10
  • IL-12 (p40)
  • IL-12 (p70)
  • IL-13
  • IL-15
  • IL-17A
  • IP-10
  • MCP-1
  • MIP-1α
  • MIP-1β
  • TNF-α
  • TNF-β
  • VEGF
  • RANTES
  • Eotaxin/CCL11
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy
  • 87% - 107%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities for individual cytokines/chemokines.
Standard Curve Range
  • 3.2–10,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HCYTMAG-60K-PX30 04055977276107

Documentation

Protocols

Title
Protocol- HCYTOMAG-60K/PX29/PMX29BK/PX30/PMX30BK/PX38/PMX38BK/PX41/PMX41BK
Protocol- HCYTOMAG-60K|HCYTMAG-60K-PX29|HCYTMAG60PMX29BK|HCYTMAG-60K-PX30|HCYTMAG60PMX30BK|HCYTMAG-60K-PX38|HCYTMAG60PMX38BK|HCYTMAG-60K-PX41|HCYTMAG60PMX41BK
Protocol: Human Cytokine / Chemokine Magnetic Bead Panel

MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel - Premixed 30 Plex - Immunology Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
Quality Control Range Sheet- HCC-121A and HCC-221A
Quality Control Ranges: MILLIPLEX® MAP Human Cytokine/ Chemokine Magnetic Bead Panel
Quality Control Ranges: Milliplex Human Cytokine / Chemokine Immunoassay