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EQCYTMG-93KPX23 MILLIPLEX MAP Equine Cytokine/Chemokine Magnetic Bead Panel Premixed 23-Plex Panel - Immunology Multiplex Assay

The Equine Inflammation / Immunology Bead-Based Multiplex Cytokine Assay kit, using the Luminex xMAP technology, enables the simultaneous analysis of 23 cytokine and chemokine biomarkers in equine serum, plasma and cell culture samples.

EQCYTMG-93KPX23
96-well plate  96 well plate
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberEQCYTMG-93KPX23
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Equine Cytokine/Chemokine Magnetic Bead Panel Premixed 23-Plex Panel - Immunology Multiplex Assay
OverviewEMD Millipore’s MILLIPLEX® MAP Equine Cytokine Magnetic Bead Premix Panel is a 23-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma or culture supernatant samples: Eotaxin/CCL11, FGF-2. Fractalkine/CS3CL1, G-CSF, GM-CSF, GRO, IFN, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8/CXCL8, IL-10, IL-12 (p70), IL-13, IL-17a, IL-18, IP-10, MCP-1, RANTES/CCL5 and TNFα.

MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.

Each MILLIPLEX® MAP panel and kit includes:
• Quality controls (QCs) provided to qualify assay performance

• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency

• Optimized serum matrix to mimic native analyte environment

• Detection antibody cocktails designed to yield consistent analyte profiles within panel


EMD Millipore’s MILLIPLEX® MAP Equine Cytokine Magnetic Bead Panel is part of the most versatile system available for complement factor research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.
Background InformationInflammation and immune cell response are key to maintaining homeostasis. The more we understand the mechanisms of disease, the more we discover the role that inflammation plays in the etiology of disease. This is a true for horses as it is for humans. Both experience immunological diseases, such as infectious disease, osteoarthritis and respiratory disease, as well as neurologic, metabolic and cardiovascular disease and even cancer. However, until now the ability to measure levels of cytokines and chemokine in horses has been limited primarily to ELISAs. However, researchers will now the ability to measure multiple inflammation biomarkers simultaneously using EMD Millipore’s MILLIPLEX ® MAP Equine Cytokine/Chemokine Panel, based on the Luminex xMAP® technology.
References
Product Information
Detection methodLuminex xMAP
ConfigurationPremixed
Precision, %
  • Inter-assay: <20
  • Intra-assay: <10
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationThe Equine Inflammation / Immunology Bead-Based Multiplex Cytokine Assay kit, using the Luminex xMAP technology, enables the simultaneous analysis of 23 cytokine and chemokine biomarkers in equine serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight (16-18 hours at 2-8 °C) or two-hour (20-25°C) incubation assay; although an overnight incubation is recommended.
• A maximum of 25 µL per well of neat serum or plasma can be used. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay.
• This kit may be used for the analysis of all of the Equine Cytokine/Chemokine Panel analytes in serum, plasma or tissue/cell/tumor culture supernatant.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Horse
Analytes Available
  • Eotaxin/CCL11
  • FGF-2
  • Fractalkine/CX3CL1
  • G-CSF
  • GM-CSF
  • GRO/KC
  • IFN-γ
  • IL-1α
  • IL-1β
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-8
  • IL-10
  • IL-12 (p70)
  • IL-13
  • IL-17A
  • IL-18
  • IP-10
  • MCP-1
  • RANTES
  • TNF-α
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy
  • Eotaxin/CCL11: 93%
  • FGF-2: 78%
  • Fractalkine/CS3CL1: 91%
  • G-CSF: 82%
  • GM-CSF: 92%
  • GRO: 97%
  • IFNγ: 83%
  • IL-1α: 89%
  • IL-1β: 88%
  • IL-2: 90%
  • IL-4: 91%
  • IL-5: 92%
  • IL-6: 76%
  • IL-8/CXCL8: 89%
  • IL-10: 86%
  • IL-12 (p70): 83%
  • IL-13: 85%
  • IL-17a: 74%
  • IL-18: 94%
  • IP-10: 86%
  • MCP-1: 90%
  • RANTES/CCL5: 88%
  • TNFα: 87%
Physicochemical Information
Sensitivity
  • Eotaxin/CCL11: 8.3 pg/mL
  • FGF-2: 16.3 pg/mL
  • Fractalkine/CS3CL1: 457 pg/mL
  • G-CSF: 340 pg/mL
  • GM-CSF: 3.94 pg/mL
  • GRO: 7.8 pg/mL
  • IFNγ: 165 pg/mL
  • IL-1α: 39.1 pg/mL
  • IL-1β: 29.3 pg/mL
  • IL-2: 13.5 pg/mL
  • IL-4: 355 pg/mL
  • IL-5: 10.6 pg/mL
  • IL-6: 1.4 pg/mL
  • IL-8/CXCL8: 74.3 pg/mL
  • IL-10: 62.5 pg/mL
  • IL-12 (p70): 49.2 pg/mL
  • IL-13: 39 – 130 pg/mL
  • IL-17A: 15.4 pg/mL
  • IL-18: 25.3 pg/mL
  • IP-10: 16.7 pg/mL
  • MCP-1: 399 ng/mL
  • RANTES/CCL5: 3.9 pg/mL
  • TNFα: 5.2 pg/mL
Standard Curve Range
  • Eotaxin/CCL11: 7 – 7,000 pg/mL
  • FGF-2: 24 – 25,000 pg/mL
  • Fractalkine/CS3CL1: 586 – 600,000 pg/mL
  • G-CSF: 78 – 80,000 pg/mL
  • GM-CSF: 5 – 5,000 pg/mL
  • GRO: 6 – 6,000 pg/mL
  • IFNγ: 488 – 500,000 pg/mL
  • IL-1α: 59 – 60,000 pg/mL
  • IL-1β: 29 – 30,000 pg/mL
  • IL-2: 15 – 15,000 pg/mL
  • IL-4: 293 – 300,000 pg/mL
  • IL-5: 15 – 15,000 pg/mL
  • IL-6: 15 – 15,000 pg/mL
  • IL-8/CXCL8: 59 – 60,000 pg/mL
  • IL-10: 49 – 50,000 pg/mL
  • IL-12 (p70): 39- 40,000 pg/mL
  • IL-13: 39 – 40,000 pg/mL
  • IL-17A: 15 – 15,000 pg/mL
  • IL-18: 34 – 35,000 pg/mL
  • IP-10: 15 – 15,000 pg/mL
  • MCP-1: 342 – 350,000 ng/mL
  • RANTES/CCL5: 5 – 5,000 pg/mL
  • TNFα: 4 - 4,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
EQCYTMG-93KPX23 04055977350128

Documentation

Protocols

Title
Protocol- EQCYTMAG-93K

MILLIPLEX MAP Equine Cytokine/Chemokine Magnetic Bead Panel Premixed 23-Plex Panel - Immunology Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Immunoassay Platform Solutions.
Custom Immunoassay Menu of Services

Technical Info

Title
96 well Template 2 Up
96 well Template Single
Quality Control Range Sheet- CYT-107 and CYT-207
Quality Control Range Sheet- CYT-108 and CYT-208
Quality Control Ranges: Milliplex® MAP Equine Cytokine/Chemokine Magnetic Bead Panel

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