Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

400051 Hygromycin B, Streptomyces sp. - CAS 31282-04-9 - Calbiochem

This product can no longer be ordered through emdmillipore.com. Please refer to (Cat. No. 400051) on sigmaaldrich.com.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Primary Target: growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells
400051
Purchase on Sigma-Aldrich

Overview

Key Spec Table

CAS #Empirical Formula
31282-04-9C₂₀H₃₇N₃O₁₃

Products

Catalogue NumberPackaging Qty/Pack
400051-1MU Alu drum 1 mu
Add To Favorites
400051-5MU Alu drum 5 mu
Add To Favorites
Description
OverviewUnique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isolated and cloned by recombinant DNA techniques. This hygromycin B-resistance gene is particularly useful for identification or selection of recombinant clones in a variety of cell types. Hygromycin B penetrates cells that have been permeabilized by virus infection and can act as an effective antiviral agent.
Bioassay: 350-450 units/mg Hygromycin B. 106 units is equivalent to ~900 mg.
Supplied with a directional insert. Note: 1 KU = 1000 units; 1 MU = 1,000,000 units.
Catalogue Number400051
Brand Family Calbiochem®
References
ReferencesDean, N. 1995. Proc. Natl. Acad. Sci. USA 92, 1287.
Hamada, W., et al. 1994. Curr. Genetics 26, 251.
Hubbard, S. C., et al. 1994. J. Biol. Chem. 269, 3717.
Rikkerink, E. H., et al. 1994. Current Genetics 25, 202.
Sugimoto, K., et al. 1994. Plant J. 5, 863.
Buchschacher, G.L., Jr., and Panganiban, A.T. 1992. J. Virol. 66, 2731.
Gaken, J., et al. 1992. Biotechniques 13, 32.
Lama, J., and Carrasco, L. 1992. J. Biol. Chem. 267, 15932.
Ma, H., et al. 1992. Gene 117, 161.
MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67.
Norman, J.A., et al. 1992. Mol. Pharmacol. 41, 53.
Bilang, R., et al. 1991. Gene 100, 247.
Dale, E., and Ow, D. 1991. Proc. Natl. Acad. Sci. USA 88, 10558.
Lee, M.G-S., and Van der Ploeg, L. 1991. Gene 105, 255.
Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol. 57, 1423.
MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother. 35, 2125.
Zhou, J., et al. 1991. Gene 107, 307.
Bulte, L., and Bennoun, P. 1990. Current Genetics 18, 155.
Giordano, T.J., and McAllister, W.T. 1990. Gene 88, 285.
Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 24, 1915.
Carrasco, L., et al. 1989. Pharmacol. Ther. 9, 311.
Crespi, C.L., et al. 1989. Carcinogenesis 10, 295.
Damm, B., et al. 1989. Mol. Gen. Genetics 217, 6.
Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9, 1965.
Kronstad, J.W., et al. 1989. Gene 79, 97.
Perlin, D.S., et al. 1988. J. Biol. Chem. 263, 118.
Cullen, D., et al. 1987. Gene 57, 21.
Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun. 117, 6.
Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
Product Information
CAS number31282-04-9
FormAmber to brown liquid
Hill FormulaC₂₀H₃₇N₃O₁₃
Chemical formulaC₂₀H₃₇N₃O₁₃
Structure formula ImageStructure formula Image
Quality LevelMQ100
Biological Information
Biological activityThe activity of Hygromycin B is measured in a zonal inhibition assay using Bacillus subtilis. The activity is measured relative to a reference standard.
Primary Targetgrowth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells
Secondary targetprotein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA
Purity>85% by HPLC
Safety Information according to GHS
RTECSWK2130000
Safety Information
R PhraseR: 26/27/28-37/38-41

Very toxic by inhalation, in contact with skin and if swallowed.
Irritating to respiratory system and skin.
Risk of serious damage to eyes.
S PhraseS: 23-26-36/37/39-45

Do not breathe fumes.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Highly Toxic & Carcinogenic / Teratogenic
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsAqueous stock solutions should be kept at concentrations of ≥50 mg/ml and can be stored at either 4°C or -20°C. Aqueous stock solutions (≥50 mg/ml) are stable for up to 6 months at 4°C or -20°C. Working solutions (< 2 mg/ml) are stable for up to 1 month at 4°C.

Note: This product is highly toxic. Read enclosed MSDS before handling.
Global Trade Item Number
Catalogue Number GTIN
400051-1MU 04055977212174
400051-5MU 07790788049966