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374805 Hemocyanin, Keyhole Limpet, Megathura crenulata

374805
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Overview

Products

Catalogue NumberPackaging Qty/Pack
374805-1GM Plastic ampoule 1 gm
374805-250MG Plastic ampoule 250 mg
Description
OverviewStable solid containing more than 60% protein. Protein is 90% undissociated hemocyanin.
Protein:≥60%; copper to protein ratio: ≥1.5 x 10-3
Catalogue Number374805
Brand Family Calbiochem®
SynonymsKLH
References
ReferencesHayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37.
Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137.
Erlanger, B.F. 1980. Methods Enzymol. 70, 85.
Product Information
CAS number9013-72-3
FormBlue to blue-gray lyophilized solid
FormulationLyophilized from BES buffer, MgSO₄.
PreservativeNone
Quality LevelMQ200
Applications
ApplicationHemocyanin, Keyhole Limpet, Megathura crenulata, CAS 9013-72-3, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.
Application CommentsProtein concentration: ≥60%
Copper: ≥0.09%
Copper to Protein ratio: ≥1.5 x 10-3
Biological Information
Purity≥90% by size exclusion chromatography
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsDissolve at 5-10 mg/ml (protein) in PBS or other physiological buffer by GENTLE addition of the buffer (e.g. slowly added down the side of the tube). DO NOT vortex or agitate vigorously to dissolve product. (KLH is an extremely large protein complex and has a tendency to aggregate and precipitate from solutions.) For further dilutions, use an appropriate buffer and maintain the magnesium concentration at ≥10 mM. Solutions should be stored in the refrigerator (4°C).
Global Trade Item Number
Catalogue Number GTIN
374805-1GM 04055977191226
374805-250MG 07790788059316