Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

110218 Gram's decolorizing solution

for the Gram staining method

Gram's decolorizing solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
110218
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
1102180500 Plastic bottle 500 ml
1102182500 Plastic bottle 2.5 l
Description
Catalogue Number110218
DescriptionGram's decolorizing solution
OverviewGram’s decolorizing solution - for the Gram staining method, is a ready-to-use solution used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution (Cat. no.109218) - an aniline dye - in the first step. After the treatment with iodine solution (Lugol’s solution stabilized with PVP, Cat no.100567), a dye-iodine complex will form. During the decolorizing step (Gram’s decolorizing solution, Cat. no. 110218), this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue-violet. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram's safranin solution (Gram's safranine solution (Cat.no. 109217) and appear pink to red.
A bottle with 500 ml Gram's decolorizing solution is sufficient for staining up to 250 slides, it is also available as part of the Gram-color stain set, (Cat. no. 111885). It is an IVD product and CE registered. For more details, please see instructions for use (IFU).
References
Product Information
HS Code3814 00 90
Quality LevelMQ400
Applications
Applicationfor the Gram staining method
Biological Information
Physicochemical Information
Density0.79 g/cm3 (20 °C)
Flash point-10 °C
Ignition temperature>425 °C
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Statement(s)H225: Highly flammable liquid and vapour.
H319: Causes serious eye irritation.
H336: May cause drowsiness or dizziness.
Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P233: Keep container tightly closed.
P240: Ground and bond container and receiving equipment.
P241: Use explosion-proof electrical/ ventilating/ lighting equipment.
P242: Use non-sparking tools.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
Signal WordDanger
Storage class3 Flammable liquids
WGKWGK 1 slightly hazardous to water
Disposal1
Strongly contaminated halogen-free organic solvents: container A.
Safety Information
Categories of dangerhighly flammable, irritant
Product Usage Statements
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RIDUN 1993 , 3, II
Declaration (transport by air) IATA-DGRUN 1993 , 3, II
Declaration (transport by sea) IMDG-CodeUN 1993 , 3, II
Supplemental Information
Specifications
Suitability for microscopypasses test
Staphylococcus aureusdark blue-violet
E.colipink to red
Global Trade Item Number
Catalogue Number GTIN
1102180500 04022536122663
1102182500 04022536557694