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361550 GSK-3 Inhibitor IX - CAS 667463-62-9 - Calbiochem

BIO, CAS 667463-62-9, is a cell-permeable, highly potent, selective, reversible, and ATP-competitive inhibitor of GSK-3α/β (IC₅₀ = 5 nM). Maintains self-renewal in human & mouse embryonic stem cells.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: BIO, (2ʹZ,3ʹE)-6-Bromoindirubin-3ʹ-oxime

Primary Target: GSK-3α/β
361550
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
667463-62-9C₁₆H₁₀BrN₃O₂

Products

Catalogue NumberPackaging Qty/Pack
361550-10MG Plastic ampoule 10 mg
361550-1MG Plastic ampoule 1 mg
361550-30MG Plastic ampoule 30 mg
Description
OverviewA cell-permeable bis-indolo (indirubin) compound that acts as a highly potent, selective, reversible, and ATP-competitive inhibitor of GSK-3α/β (IC50 = 5 nM). Its specificity has been tested against various Cdk's (IC50 = 83, 300, 320, and 10,000 nM for Cdk5/p25, Cdk2/A, Cdk1/B, and Cdk4/D1, respectively) as well as many other commonly studied kinases (IC50 ≥ 10 µM), including MAP kinases, PKA, PKC isoforms, PKG, CK, and IRTK. Inhibition of GSK by BIO has been shown to result in the activation of Wnt-signaling pathway and sustained pluripotency in human and murine ESCs (embryonic stem cells). Reported to maintain self-renewal in human and mouse embryonic stem cells. Also induces the differentiation of neonatal cardiomyocytes. A 10 mM (500 µg/140 µl) solution of GSK-3 Inhibitor IX (Cat. No. 361552) in DMSO is also available.
Catalogue Number361550
Brand Family Calbiochem®
SynonymsBIO, (2ʹZ,3ʹE)-6-Bromoindirubin-3ʹ-oxime
References
ReferencesTseng, A-S., et al. 2006. Chem. Biol. 13, 957.
Polychronopoulos, P., et al. 2004. J. Med. Chem. 47, 935.
Sato, N., et al. 2004. Nat. Med. 10, 55.
Meijer, L., et al. 2003. Chem. Biol. 10, 1255.
Product Information
CAS number667463-62-9
ATP CompetitiveY
FormPurple solid
Hill FormulaC₁₆H₁₀BrN₃O₂
Chemical formulaC₁₆H₁₀BrN₃O₂
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetGSK-3α/β
Primary Target IC<sub>50</sub>5 nM against GSK-3α/β
Purity≥97% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 20/21/22

Harmful by inhalation, in contact with skin and if swallowed.
S PhraseS: 22-36/37

Do not breathe dust.
Wear suitable protective clothing and gloves.
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Carcinogenic / Teratogenic
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
361550-10MG 04055977214079
361550-1MG 04055977214062
361550-30MG 04055977214086

Documentation

GSK-3 Inhibitor IX - CAS 667463-62-9 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

GSK-3 Inhibitor IX - CAS 667463-62-9 - Calbiochem Certificates of Analysis

TitleLot Number
361550

References

Reference overview
Tseng, A-S., et al. 2006. Chem. Biol. 13, 957.
Polychronopoulos, P., et al. 2004. J. Med. Chem. 47, 935.
Sato, N., et al. 2004. Nat. Med. 10, 55.
Meijer, L., et al. 2003. Chem. Biol. 10, 1255.

Citations

Title
  • Anthony R. White, et al. (2006) Degradation of the alzheimer disease amyloid -peptide by metal-dependent up-regulation of metalloprotease activity. journal of Biological Chemistry 281, 17670-17680.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision03-April-2008 RFH
    SynonymsBIO, (2ʹZ,3ʹE)-6-Bromoindirubin-3ʹ-oxime
    DescriptionA cell-permeable, highly potent, selective, reversible, and ATP-competitive inhibitor of GSK-3α/β (IC50 = 5 nM). Specificity was confirmed using various Cdk's (IC50 = 83 nM for Cdk5/p25, 300 nM for Cdk2/cyclin A, 320 nM for Cdk1/cyclin B, and 10 µM for Cdk4/cyclin D1), MAP kinases, PKA, PKC isoforms, PKG, CK, and IRTK (IC50 ≥10 µM). Inhibition of GSK by BIO has been shown to result in the activation of the Wnt signaling pathway and sustained pluripotency of human and murine embryonic stem cells (ESCs). Reported to maintain self-renewal in human and mouse embryonic stem cells. Also induces the differentiation of neonatal cardiomyocytes.
    FormPurple solid
    Intert gas (Yes/No) Packaged under inert gas
    CAS number667463-62-9
    Chemical formulaC₁₆H₁₀BrN₃O₂
    Structure formulaStructure formula
    Purity≥97% by HPLC
    SolubilityDMSO (5 mg/ml)
    Storage Protect from light
    +2°C to +8°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
    Toxicity Carcinogenic / Teratogenic
    ReferencesTseng, A-S., et al. 2006. Chem. Biol. 13, 957.
    Polychronopoulos, P., et al. 2004. J. Med. Chem. 47, 935.
    Sato, N., et al. 2004. Nat. Med. 10, 55.
    Meijer, L., et al. 2003. Chem. Biol. 10, 1255.
    Citation
  • Anthony R. White, et al. (2006) Degradation of the alzheimer disease amyloid -peptide by metal-dependent up-regulation of metalloprotease activity. journal of Biological Chemistry 281, 17670-17680.
    		•