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Filtration Discs

Membranes for use with stirred cells

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Overview

Specifications

Ordering Information

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Ultracel® PL-1 Membrane, 1,000 MWCOClear Sorting & Filtering Show Filter
Catalogue NumberFilter Diameter (⌀)Pack Size
PLAC0251025 mm 10
PLAC0431044.5 mm 10
PLAC0471047 mm 10
PLAC0621063.5 mm 10
PLAC0761076 mm 10
PLAC0900590 mm 5
PLAC15005150 mm 5

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Ultracel® PL-3 Membrane, 3,000 MWCOClear Sorting & Filtering Show Filter
Catalogue NumberFilter Diameter (⌀)Pack Size
PLBC0251025 mm 10
PLBC0431044.5 mm 10
PLBC0471047 mm 10
PLBC0621063.5 mm 10
PLBC0761076 mm 10
PLBC0900590 mm 5
PLBC15005150 mm 5

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Ultracel® PL-5 Membrane, 5,000 MWCOClear Sorting & Filtering Show Filter
Catalogue NumberFilter Diameter (⌀)Pack Size
PLCC0251025 mm 10
PLCC0431044.5 mm 10
PLCC0471047 mm 10
PLCC0621063.5 mm 10
PLCC0761076 mm 10
PLCC0900590 mm 5
PLCC15005150 mm 5

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Ultracel® PL-10 Membrane, 10,000 MWCOClear Sorting & Filtering Show Filter
Catalogue NumberFilter Diameter (⌀)Pack Size
PLGC0251025 mm 10
PLGC0431044.5 mm 10
PLGC0471047 mm 10
PLGC0621063.5 mm 10
PLGC0761076 mm 10
PLGC0900590 mm 5
PLGC15005150 mm 5

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Ultracel® PL-30 Membrane, 30,000 MWCOClear Sorting & Filtering Show Filter
Catalogue NumberFilter Diameter (⌀)Pack Size
PLTK0251025 mm 10
PLTK0431044.5 mm 10
PLTK0471047 mm 10
PLTK0621063.5 mm 10
PLTK0761076 mm 10
PLTK0900590 mm 5
PLTK15005150 mm 5

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Ultracel® PL-100 Membrane, 100,000 MWCOClear Sorting & Filtering Show Filter
Catalogue NumberFilter Diameter (⌀)Pack Size
PLHK0251025 mm 10
PLHK0431044.5 mm 10
PLHK0471047 mm 10
PLHK0621063.5 mm 10
PLHK0761076 mm 10
PLHK0900590 mm 5
PLHK15005150 mm 5

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Biomax® Membrane, 5,000 MWCOClear Sorting & Filtering Show Filter
Catalogue NumberFilter Diameter (⌀)Pack Size
PBCC0251025 mm 10
PBCC0431044.5 mm 10
PBCC0471047 mm 10
PBCC0621063.5 mm 10
PBCC0761076 mm 10

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Biomax® Membrane, 10,000 MWCOClear Sorting & Filtering Show Filter
Catalogue NumberFilter Diameter (⌀)Pack Size
PBGC0251025 mm 10
PBGC0431044.5 mm 10
PBGC0471047 mm 10
PBGC0621063.5 mm 10
PBGC0761076 mm 10

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Biomax® Membrane, 30,000 MWCOClear Sorting & Filtering Show Filter
Catalogue NumberFilter Diameter (⌀)Pack Size
PBTK0251025 mm 10
PBTK0431044.5 mm 10
PBTK0471047 mm 10
PBTK0621063.5 mm 10
PBTK0761076 mm 10

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Biomax® Membrane, 50,000 MWCOClear Sorting & Filtering Show Filter
Catalogue NumberFilter Diameter (⌀)Pack Size
PBQK0251025 mm 10
PBQK0431044.5 mm 10
PBQK0471047 mm 10
PBQK0621063.5 mm 10
PBQK0761076 mm 10

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Biomax® Membrane, 100,000 MWCOClear Sorting & Filtering Show Filter
Catalogue NumberFilter Diameter (⌀)Pack Size
PBHK0251025 mm 10
PBHK0431044.5 mm 10
PBHK0471047 mm 10
PBHK0621063.5 mm 10
PBHK0761076 mm 10

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Biomax® Membrane, 300,000 MWCOClear Sorting & Filtering Show Filter
Catalogue NumberFilter Diameter (⌀)Pack Size
PBMK0251025 mm 10
PBMK0431044.5 mm 10
PBMK0471047 mm 10
PBMK0621063.5 mm 10
PBMK0761076 mm 10
PBMK0900590 mm 5
PBMK15005150 mm 5

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Biomax® Membrane, 500,000 MWCOClear Sorting & Filtering Show Filter
Catalogue NumberFilter Diameter (⌀)Pack Size
PBVK0251025 mm 10
PBVK0431044.5 mm 10
PBVK0471047 mm 10
PBVK0621063.5 mm 10
PBVK0761076 mm 10

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0.1um Hydrophilic Durapore®Clear Sorting & Filtering Show Filter
Catalogue NumberFilter Diameter (⌀)Pack Size
VVLP0622563.5 mm PVDF, Hydrophilic, 0.1 µm, 63.5 mm, white, plain, 25
VVLP0762576 mm PVDF, Hydrophilic, 0.1 µm, 76 mm, white, plain, 25

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0.22um Hydrophilic Durapore®Clear Sorting & Filtering Show Filter
Catalogue NumberFilter Diameter (⌀)Pack Size
GVWP0622563.5 mm PVDF, Hydrophilic, 0.22 µm, 63.5 mm, white, plain, 25
GVWP0762576 mm PVDF, Hydrophilic, 0.22 µm, 76mm, white, plain, 25

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0.45um Hydrophilic Durapore®Clear Sorting & Filtering Show Filter
Catalogue NumberFilter Diameter (⌀)Pack Size
HVLP0622563.5 mm PVDF, Hydrophilic, 0.45 µm, 63.5 mm, white, plain, 25
HVLP0762576 mm PVDF, Hydrophilic, 0.45 µm, 76mm, white, plain, 25

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Stirred Cell AccessoriesClear Sorting & Filtering Show Filter
Catalogue Number
6003
6028
6015

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Documentation

References

Reference overviewApplication
MicroRNA expression detected by oligonucleotide microarrays: System establishment and expression profiling in human tissues
Omer Barad, Eti Meiri, Amir Avniel, Ranit Aharonov, Adi Barzilai, Isaac Bentwich, Uri Einav, Shlomit Gilad, Patrick Hurban, Yael Karov, Edward K. Lobenhofer, Eilon Sharon, Yoel M. Shiboleth, Marat Shtutman, Zvi Bentwich and Paz Einat
Genome Research 14:2486-2494, 2004  2004

Highly selective membranes in protein ultrafiltration
Feins M, Sirkar KK,Biotechnol Bioeng. 2004 Jun 20;86(6):603-11
Biotechnol Bioeng. 2004 Jun 20;86(6):603-11  2004

Protein Purification
Electrokinetic characterisation of ultrafiltration membranes by streaming potential, electroviscous effect, and salt retention
Huisman IH (REPRINT) ; Pradanos P; Hernandez A
Journal of membrane science 2000, V178, N1-2, p55-64 2000  2000

Effect of Solution pH on Protein Transport Through Ultrafiltration Membranes
Douglas B. Burns and Andrew L. Zydney
Biotechnol Bioeng 64: 27-37, 1999.  1999

Integrated process for the removal of emulsified oils from effluents in the steel industry
Benito JM; Rios G; Gutierrez B; Pazos C; Coca J (REPRINT)
Separation Science and Technology 1999, V34, N15, P3031-3043 SN- 0149-6395  1999

Industrial QC
Performance Characteristics of Waste Oil Emulsion Treatment by Ultrafiltration
Lin, S. H.; Lan, W. J. Yuan Ze University, Taoyuan, Taiwan
J Environ Sci Health-Toxic Hazard Subst Environ Eng 1998,SO- vA33, n3, p385(20)  1998

General approach for the development of high-performance liquid chromatography methods for biosurfactant analysis and purification
Lin, S.-C. Chen, Y.-C. Lin, Y.-M.
J. Chromatogr., A ; Journal of Chromatography, A v. 825 pg 149-159 6 Nov 1998  1998

Filtration 15 nm: Aspects Techniques
Chtourou S,Sang thrombose vaisseaux, 21-27, 1998
Sang thrombose vaisseaux, 21-27, 1998  1998

Distribution of trace elements associated with dissolved compounds (<0.45 .mu.m-1 nm) in freshwater using coupled (frontal cascade) ultrafiltration and chromatographic separations
Pham, M. K. Garnier, J.-M.
Environmental Science and Technology, v32, pg. 440-449, 15 Feb. 1998  1998

Acrylamide production in an ultrafiltration-membrane bioreactor using cells of Brevibacterium imperialis CBS 489-74
Cantarella M (reprint) ; Spera A; Cantarella L; Alfani F ; Journal of membrane science, v147, N2, p279-290 1998
Journal of membrane science, v147, N2, p279-290 1998  1998

Enzyme Assays
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FAQ

QuestionAnswer
Are there any extractables in the UF disc membranes?The YM, PM and XM membranes are treated with glycerin as a humectant. The glycerin bath contains sodium azide as a preservative. The membranes contain no other extractables.
As a rule of thumb, when separating two globular solutes, what size difference is required when performing ultrafiltration?In general, a 10 fold difference is recommended.
Describe what happens when albumin (67,000 MW) and IgG (167,000 MW) are filtered through a 100,000 MW cut-off membrane?The larger solute will form a gel layer preventing the smaller solute from completely passing. If processed separately, the IgG would be retained and the albumin would pass through the membrane.
How can I clean and restore the Ultracel and Biomax membranes after use?Rinse the membrane for 30 minutes with either 0.1 M NaOH, 100 ppm NaOCl (at 25 degrees C), or 0.1 - 0.5% chemical cleaner. After rinsing, flush the membrane thoroughly with distilled or deionized water.
How effective is ultrafiltration for fractionation of protein solutions?Ultrafiltration has limited utility for protein fractionation. Pore size distribution and membrane polarization effects contribute to the lack of separation precision. This is the reason for the wide spacing given to molecular weight cutoff points. Generally, an order of magnitude (10x) difference in molecule size is required to achieve reasonable separation. Separations may be enhanced by reducing the protein concentration, increasing agitation, or crossflow rate, or by optimizing buffers to minimize protein aggregation and adsorption.
Is the Ultracel YM membrane autoclavable?Yes, the Ultracel YM membrane can be autoclaved, submerged in water, at 121 degrees C.
Which UF membrane do I want, Biomax or Ultracel?Biomax is recommended for applications where very harsh pH conditions are required for processing or cleaning. Ultracel is recommended where harsh pH conditions are not needed and especially when protein loading is low (<20-g/m sq.) or the feedstock is highly fouling. Biomax is currently the premier UF membrane being used in Life Science applications, primarily due to its combination of low protein binding (i.e., high yield) and speed. Ultracel, Millipore’s new composite regenerated cellulose offering, is the lowest protein binding UF membrane available, making it ideal for dilute protein solutions where binding may be a significant factor in yield. For important applications, such as pilot trials prior to scale-up, it is recommended to conduct a trial with several pore sizes from each membrane family in order to determine which best meets the application criteria.
When doing diafiltration, how many initial volumes would it take to remove the salt?It takes three initial volumes to remove 95% of the salt and five initial volumes to remove 99.9% of the salt.
What molecular weight cut-off should I select for proteins of different sizes?As a rule, when using Ultracel membranes it is best to select a membrane 2-3 times smaller than the solute to be retained by the membrane. In the case of the Biomax membranes 4-5 times smaller than the NMWL is suggested. For more information on choosing the appropriate membrane cutt off: http://www.millipore.com/publications.nsf/docs/RF036
What is the best way to store disc membranes?Solutions of 10% ethanol in water or 0.1% azide in water will preserve the membrane. For long-term storage, refrigeration is recommended.
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