Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

GF003AF-MG Fibroblast Growth Factor basic Protein, Human recombinant (mg qty.)

The Fibroblast Growth Factor-basic (or bFGF protein) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal & endothelial cells.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
UniProt Number: P09038 Entrez Gene Number: NM_002006.4
GF003AF-MG
1 mg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

SpeciesSourcePurity
Human expressed in E. coliGreater than 95% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg of bFGF.
Description
Catalogue NumberGF003AF-MG
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionFibroblast Growth Factor basic Protein, Human recombinant (mg qty.)
Alternate Names
  • FGF-2
  • bFGF
  • basic-FGF
  • Heparin-Binding Growth Factor 2
  • Prostatropin
Background InformationFibroblast Growth Factor-basic (bFGF) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 17.2 kDa protein containing 155 amino acid residues. Xu, et al. demonstrated that bFGF synergizes with the BMP antagonist noggin to sustain undifferentiated proliferation of human embryonic stem (hES) cells under feeder-free conditions. GF003AF was developed without animal-based ingredients and can be used for the culture of hES cells in a feeder-free, animal-free culture system.
References
Product Information
HS Code2937 19 90
PresentationLyophilized from a solution of 5mM Tris, pH 7.6 with 150 mM NaCl.
Quality LevelMQ100
Applications
ApplicationThe Fibroblast Growth Factor-basic (or bFGF protein) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal & endothelial cells.
Application NotesFor most in vitro applications, bFGF exerts its biological activity in the concentration range of 0.1 to 10.0 ng/mL. Responding cells are (partial list): Endothelial, mesenchymal cells. Human ES cells require concentrations in the range of 4 to 100 ng/ml, depending on the method of culture
Biological Information
PurityGreater than 95% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg of bFGF.
Sourceexpressed in E. coli
Specific ActivityChemicon's Human FGF-b is fully biologically active when compared to standards. The ED<sub>50</sub> was determined by the dose-dependent stimulation of thymidine uptake by NIH3T3 cells expressing FGF receptors.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
Gene Symbol
  • FGF2
  • prostatropin
  • Prostatropin
  • HBGF-2
  • BFGF
  • FGFB
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P09038 # The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
SIZE: 155 amino acids; 17254 Da
SUBUNIT: Monomer. Interacts with CSPG4 and FGFBP1. Found in a complex with FGFBP1, FGF1 and FGF2.
SIMILARITY: SwissProt: P09038 ## Belongs to the heparin-binding growth factors family.
MISCELLANEOUS: This protein binds heparin more strongly than does aFGF.
Stem Cell Type
  • Neural Stem Cells
  • Epithelial Cells
  • Mesenchymal Stem Cells
  • Pancreatic Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain the lyophilized material at -20°C until expiration date as stated on the label.

General applications:
After a quick spin, reconstitute in 0.1M phosphate buffer, pH 6.8, to a concentration of 0.1-1.0 mg/mL. Reconstituted bFGF should be stored in working aliquots at -20°C for up to six months. Multiple freeze/thaw cycles will result in significant loss of activity.

For Human ES cell culture:
After a quick spin, reconstitute to 10 μg/mL in a filtered solution of 0.5% BSA, 1 mM DTT, and 10% glycerol in Dulbecco's PBS. Aliquot and store at -20°C for up to six months. This solution can then thawed and diluted to 4 ng/mL for the culture of human ES cells with a feeder layer, or to 8 ng/mL to supplement mouse embryonic fibroblast-conditioned medium (for feeder-free human ES cell culture).
Packaging Information
Material Size1 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GF003AF-MG 04053252326578

Documentation

Fibroblast Growth Factor basic Protein, Human recombinant (mg qty.) SDS

Title

Safety Data Sheet (SDS) 

Fibroblast Growth Factor basic Protein, Human recombinant (mg qty.) Certificates of Analysis

TitleLot Number
RECOMBINANT ANIMAL-FREE HUMAN FIBROBLAST GROWTH FACTOR-BASIC - 2330574 2330574
RECOMBINANT ANIMAL-FREE HUMAN FIBROBLAST GROWTH FACTOR-BASIC - 2390531 2390531
RECOMBINANT ANIMAL-FREE HUMAN FIBROBLAST GROWTH FACTOR-BASIC - 2455620 2455620
RECOMBINANT ANIMAL-FREE -2508896 2508896
RECOMBINANT ANIMAL-FREE -2700962 2700962
RECOMBINANT ANIMAL-FREE -2746009 2746009
RECOMBINANT ANIMAL-FREE HUMAN FIBROBLAST GROWTH FACTOR-BASIC 2934834
RECOMBINANT ANIMAL-FREE HUMAN FIBROBLAST GROWTH FACTOR-BASIC 2474982
RECOMBINANT ANIMAL-FREE HUMAN FIBROBLAST GROWTH FACTOR-BASIC 2846668
RECOMBINANT ANIMAL-FREE HUMAN FIBROBLAST GROWTH FACTOR-BASIC - 1992395 1992395
Show more

References

Reference overviewPub Med ID
Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells
Xu, Ren-He, et al
Nat Methods, 2:185-190 (2005)  2005

15782187 15782187

Data Sheet

Title
RECOMBINANT ANIMAL-FREE HUMAN FIBROBLAST GROWTH FACTOR-BASIC
Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation