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324717 Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli

324717
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Overview

Products

Catalogue NumberPackaging Qty/Pack
324717-200MIU Plastic ampoule 200 miu
Description
OverviewRecombinant, streptomyces plicatus endoglycosidase H expressed in E. coli. Catalyzes the hydrolysis of asparagine-linked oligomannose and hybrid, but not complex oligosaccharides from glycoprotein. Endo H cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.
Note: 1 mU = 1 milliunit.
Catalogue Number324717
Brand Family Calbiochem®
SynonymsEndo-β-N-acetylglucosaminidase H, Endo H
References
ReferencesRao, V., et al. 1995. Structure 3, 449.
Trimble, R.B., et al. 1987. Methods Enzymol. 138, 763.
Trumbly, R.J., et al. 1985. J. Biol. Chem. 260, 5683.
Product Information
CAS number52769-51-4
Activity≥3 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5. Cleavage is monitored by SDS-PAGE.
EC number3.2.1.96
FormLiquid
FormulationIn 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.
Quality LevelMQ100
Biological Information
Specific Activity≥30 units/mg protein
Physicochemical Information
ContaminantsN-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases: none detected
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Global Trade Item Number
Catalogue Number GTIN
324717-200MIU 04055977215984