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PMEF-NL EmbryoMax® Primary Mouse Embryonic Fibroblasts

PMEF, Neo Resistant, Strain FVB, Not Treated, Passage 3

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture
PMEF-NL
5 vials  5-6x106 ea
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Key Spec Table

Key Applications
Cult
Description
Catalogue NumberPMEF-NL
Trade Name
  • Embryomax
  • Specialty Media
DescriptionEmbryoMax® Primary Mouse Embryonic Fibroblasts
OverviewPrimary Mouse Embryo Fibroblasts, Neo resistant, strain FVB, are not Mytomycin C treated and serve as a feeder layer for both mouse and human ES/IPS cells. MEF cells are resistant to neomycin. The cells are provided at passage 3 and can be further expanded before freezing for later use.

Plating MEF Feeder Cells

Procedure:

1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution.
2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes.
3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium.
4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide
5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density and
appearance. Gelatinized plates may be used for 12–14 days.
Alternate Names
  • MEF Feeder Cells, neo PMEFs, neo MEFs, MEFs, Mouse Feeder Cells
Background InformationThe EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.
Product Information
PresentationThe cells are provided frozen into individual vials, containing approximately 5-6 x 106 fibroblasts per vial, and are sold as a pack of 5 vials and supplied at passage 3.
Quality LevelMQ100
Applications
ApplicationPMEF, Neo Resistant, Strain FVB, Not Treated, Passage 3
Key Applications
  • Stem Cell Culture
Biological Information
Stem Cell Type
  • Mouse Embryonic Stem Cells
  • PMEFs
  • Induced Pluripotent Stem Cells
Cell Line Type
  • Primary Mouse Embryo Fibroblasts
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsAfter receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.
Packaging Information
Material Size5 vials
Material Package5-6x106 ea
Global Trade Item Number
Catalogue Number GTIN
PMEF-NL 04053252317163