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251760 DAR-4M - Calbiochem

A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.

DAR-4M - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Diaminorhodamine-4M

251760
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Empirical Formula
C₂₅H₂₆N₄O₃

Products

Catalogue NumberPackaging Qty/Pack
251760-1MG Plastic ampoule 1 mg
Description
OverviewA rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of O2, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH, unlike DAF-2 (Cat. No. 251500).
Catalogue Number251760
Brand Family Calbiochem®
SynonymsDiaminorhodamine-4M
References
ReferencesKojima, H., et al. 2001. Anal. Chem. 73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett. 41, 69.
Product Information
ATP CompetitiveN
DeclarationNot available for sale in Japan.
FormRed violet solution
FormulationIn DMSO.
Hill FormulaC₂₅H₂₆N₄O₃
Chemical formulaC₂₅H₂₆N₄O₃
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetNitric oxide (NO) fluorescent indicator
Purity≥98% by HPLC
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 23-26-36

Do not breathe fumes.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze at (-20°C). Use immediately following dilution and discard any unused portion.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
251760-1MG 04055977217254

Documentation

DAR-4M - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

DAR-4M - Calbiochem Certificates of Analysis

TitleLot Number
251760

References

Reference overview
Kojima, H., et al. 2001. Anal. Chem. 73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett. 41, 69.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-December-2018 JSW
SynonymsDiaminorhodamine-4M
DescriptionA rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. Unlike DAF-2 (Cat. No. 251500), its fluorescence intensity is not dependent on the pH. Excitation maximum: ~560 nm; emission maximum: ~575 nm.
FormRed violet solution
FormulationIn DMSO.
Recommended reaction conditionsThis reagent is provided as a solution at 5 mM concentration. Prepare a 500-fold dilution in 0.1 M phosphate buffer, pH 7.4 before use. BSA and phenol-red may affect fluorescence.
Chemical formulaC₂₅H₂₆N₄O₃
Structure formulaStructure formula
Purity≥98% by HPLC
Storage Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze at (-20°C). Use immediately following dilution and discard any unused portion.
Toxicity Irritant
ReferencesKojima, H., et al. 2001. Anal. Chem. 73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett. 41, 69.