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CC319 CytoSoft® Imaging 96-Well Plate

Elastic Moduli 64 kPa

CC319
1 Plate  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberCC319
DescriptionCytoSoft® Imaging 96-Well Plate
OverviewThe rigidity of the cell’s substrate (such as the extracellular matrix) can have a profound effect on cell function including morphology, differentiation and gene expression. Young's elastic modulus is a mechanical property that measures the stiffness of a solid material. It defines the relationship between stress and strain in a material and is measured in kPa.

CytoSoft® plates provide a tool to culture cells on substrates with defined rigidities covering a broad physiological range (0.2 kPa- 64 kPa). On the bottom of each well, there is a thin layer of specially formulated biocompatible silicone, whose elastic modulus (rigidity) is carefully measured and certified. The surfaces of the gels are functionalized to form covalent bonds with amines on proteins. The gel readily binds to purified ECM proteins, such as PureCol® type I collagen (5006), prior to cell addition.

CytoSoft® Imaging plates are designed for high-resolution imaging where low autofluorescence and exceptional optical clarity are required. These plates accommodate both live cell imaging and common fixation techniques such as immunostaining. Plate consists of a 175 µm thin polycarbonate film-bottom plate bonded with a black polystyrene frame and includes a lid. The plates are sterilized using ozone and provided with 1 plate per package.

Instructions For Use

Coating Procedure

Note: Use these recommendations as guidelines to determine the optimal coating conditions for your culture system. The bottom of the plate can be detached by excessive mechanical force such as centrifugation or direct contact with liquid handling tools (tips, pipettes, etc.).

1. Remove the CytoSoft® product from the protective sleeve in a sterile hood.
2. Prepare extracellular matrix material by neutralizing in amine-free buffer pH 7.4 to 7.9 (such as 1X DPBS). We do not recommend using gelatin as your ECM protein. Note: Pre-warm the coating solution to approximately room temperature before use.
3. Dilute as needed, and dispense 150 µL of solution into each well to coat the surface. Note: Recommended dilution for PureCol® Type I collagen is 1:30 (~100 µg/ml). Note: The hydrophobic surface requires larger volumes to cover the surface than do conventional plastic dishes
4. Incubate ECM coated CytoSoft® at room temperature, covered for 1 hour.
5. After incubation, aspirate any remaining material and rinse coated surfaces immediately two times with culture medium or PBS. Leave about 150 µL of medium per well to keep surface covered. Note: Do not allow the CytoSoft® surface to become dry once the surface has been wetted.
6. Coated surfaces are ready for use. Standard harvesting procedures used for removing cells from cultureware can be employed for harvesting cells from the CytoSoft® product including use of trypsin, Accutase® and non-enzymatic cell detachment solutions.

CytoSoft® is a registered trademark of Advanced BioMatrix, Inc.
References
Product Information
Components
  • 1 CytoSoft® Imaging 96-Well Plate
Device Configuration96-well plate
Applications
ApplicationElastic Moduli 64 kPa
Key Applications
  • Cell Culture
Biological Information
SterilityOther
Physicochemical Information
Recommended Media Volume
40 to 280 μL
Dimensions
Surface Area0.32 cm2
Materials Information
Device Material
  • Silicone
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceYoung’s elastic modulus: 52.0-74.0 kPa
Sterility: Ozone
Cell attachment: Tested
Cell growth: Tested
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore CytoSoft® Imaging plates at room temperature.
Packaging Information
Material Size1 Plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CC319 04061841868866