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S7865 CpGenome™ Rat Methylated and Unmethylated Genomic DNA Standard Set

Useful as a control or standard for variety of epigenomic assays such as methylated or unmethylated DNA enrichment, bisulfite conversion or melt curve analysis to determine the status of global or locus- specific DNA methylation.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
S7865
5 µg per vial  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberS7865
Brand Family Upstate
Trade Name
  • CpGenome
  • Upstate
DescriptionCpGenome™ Rat Methylated and Unmethylated Genomic DNA Standard Set
OverviewThe CpGenome™ Rat Methylated and Unmethylated Genomic DNA Standard Set contains one vial of unmethylated DNA and one vial of methylated DNA generated from the same genomic DNA source.

The CpGenome™ Rat Unmethylated Genomic DNA Standard is generated using multiple strand displacement amplification of rat genomic DNA using Phi29 DNA polymerase. This process results in a DNA standard which contains ≥ 95% unmethylated cytosines across the genome in both heterochromatic and euchromatic regions.

The CpGenome™ Rat Methylated Genomic DNA Standard is generated using multiple strand displacement amplification of rat genomic DNA using Phi29 DNA polymerase followed by a treatment with a CpG Methylase. This process results in a DNA standard which contains ≥ 95% methylated cytosines across the genome in both heterochromatic and euchromatic regions.
References
Product Information
HS Code2934 99 99
Presentation5 µg DNA in buffer containing 10mM Tris-HCl pH 8.0, 0.1mM EDTA
Quality LevelMQ100
Applications
ApplicationUseful as a control or standard for variety of epigenomic assays such as methylated or unmethylated DNA enrichment, bisulfite conversion or melt curve analysis to determine the status of global or locus- specific DNA methylation.
Biological Information
Species Reactivity
  • Rat
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceQuantitative Methylation Specific PCR (qMSP):
Bisulfite converted unmethylated and methylated rat genomic DNA are assessed for methylation status at various loci within the genome.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsUpon receipt aliqout and store at -20°C to avoid multiple freeze thaws which can fragment DNA. For best quality, limit freeze and thaw of the same –20°C DNA aliquot to no more than 3 times. For longer term storage, the DNA can be stored at -80°C. With proper storage and protection from excess freeze-thaw cycles, this product is stable for up to two years from the date of receipt.
Packaging Information
Material Size5 µg per vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
S7865 04055977118216

Documentation

CpGenome™ Rat Methylated and Unmethylated Genomic DNA Standard Set SDS

Title

Safety Data Sheet (SDS)