Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

234401 Complement C1q-Depleted Serum, Human

Complement C1q-Depleted Serum, Human MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
234401
Purchase on Sigma-Aldrich

Overview

Products

Catalogue NumberPackaging Qty/Pack
234401-1ML Plastic ampoule 1 ml
Add To Favorites
Description
OverviewHuman serum that has been depleted of C1q complement component.
Catalogue Number234401
Brand Family Calbiochem®
Product Information
Unit of Definition<b>Classical Pathway Activity:</b> One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 10⁷ EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++. <b>CH50 Functional Activity upon Reconstitution:</b> One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 10⁸ EA when incubated for 60 min at 37°C in a total reaction volume of 1.5 ml GVB++. <b>Alternative Pathway Activity:</b> One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 10⁷ rabbit erythrocytes when incubated for 30 min at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM.
FormClear, amber solution
FormulationIn PBS, ≤0.1% sodium azide, pH 7.4.
PreservativeNone
Quality LevelMQ100
Applications
Application CommentsNo C1q detected by Ouchterlony using various dilutions of goat anti-human C1q antiserum. The following information will vary from lot to lot and can be found on the certificate of analysis:

CLASSICAL PATHWAY ACTIVITY: One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 107 EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 minutes at 37°C in a total reaction volume of 500 μl GVB++.
• Recommended volume of C1q-Dpl serum per assay
• C1qH50 units/mg purified C1q at the recommended input of C1q-Dpl
• Input of purified C1q to yield 1 C1qH50:
• C1qH50 units/ml NHS Complement standard at the recommended input of C1q-Dpl
• Input of C1q in NHS Complement standard to yield 1 C1qH50
• Ratio
• Background A412 EA blank reading at the recommended input of C1q-Dpl

CH50 FUNCTIONAL ACTIVITY UPON RECONSTITUTION: One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 108 EA when incubated for 60 minutes at 37°C in a total reaction volume of 1.5 ml GVB++.
• CH50/ml NHS Standard
• CH50/ml C1q-Dpl reconstituted with 100 μg C1q/ml:
• Ratio

ALTERNATIVE PATHWAY ACTIVITY: One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 107 rabbit erythrocytes when incubated for 30 minutes at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM.
• APH50/ml NHS Standard
• Input of NHS Standard to yield 1 APH50
• APH50/ml C1q-Dpl
• Input of C1q-Dpl to yield 1 APH50
• AP Ratio
Biological Information
SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
ContaminantsC1q: No C1q detected by Ouchterlony using various dilutions of goat anti-human C1q antiserum.
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Global Trade Item Number
Catalogue Number GTIN
234401-1ML 04055977200195