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HTS005M ChemiSCREEN™ CCR1 Membrane Preparation

Human CCR1 GPCR membrane preparation for Radioligand binding Assays.

ChemiSCREEN™ CCR1 Membrane Preparation MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-2 Uni Prot Number: P32246 Entrez Gene Number: NM_001295.2
HTS005M
1 mL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

SpeciesHost CellsProtein Target
Human Chem-2CCR1
Description
Catalogue NumberHTS005M
Brand Family Chemicon®
Trade Name
  • ChemiScreen
  • Chemicon
DescriptionChemiSCREEN™ CCR1 Membrane Preparation
OverviewFull-length human CCR1 cDNA
Background InformationCCR1 is a GPCR that binds to a variety of CC ligands, including MIP-1a, RANTES, MCP-3, HCC-1, HCC-2, HCC-4, and MPIF-1 (Olson and Ley, 2002). Lymphocytes, macrophages, dendritic cells, and GM-CSF-activated neutrophils express CCR1 (Kaufmann et al., 2001; Cheng et al., 2001). Two selective, non-peptide small molecule antagonists of CCR1, BX-471 and CP-481,715, have been synthesized (Gladue et al., 2003; Liang et al., 2000). Pharmacological and genetic targeting of CCR1, either alone or in combination with cyclosporin A, reduces cardiac and renal allograft rejection (Gao et al., 2000; Horuk et al., 2001a; Horuk et al., 2001b), allergic encephalomyelitis (Liang et al., 2000), and renal fibrosis (Anders et al., 2002) in experimental models. Chemicon's CCR1 Membrane Preparations are ideal for screening molecules that disrupt interaction between CCR1 and its ligands. The CCR1 Membrane Preps bind to MIP-1a with a Kd of 1.8 nM, and yield greater than 10-fold signal to background ratio with a MIP-1a concentration of 0.1 nM.
References
Product Information
FormatMembranes
PresentationLiquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.
Each vial typically contains 500 units, where one unit is the amount of membrane prep that yields greater than 1000 cpm specific binding of 125I-labeled MIP-1α (0.1 nM input concentration).
Quality LevelMQ100
Applications
ApplicationHuman CCR1 GPCR membrane preparation for Radioligand binding Assays.
Key Applications
  • Radioligand binding assay
Application NotesRadioligand binding assay.
Biological Information
Concentration0.5 mg/mL
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The ligands of this receptor include macrophage inflammatory protein 1 alpha (MIP-1 alpha), regulated on activation normal T expressed and secreted protein (RANTES), monocyte chemoattractant protein 3 (MCP-3), and myeloid progenitor inhibitory factor-1 (MPIF-1). Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. Knockout studies of the mouse homolog suggested the roles of this gene in host protection from inflammatory response, and susceptibility to virus and parasite. This gene and other chemokine receptor genes, including CCR2, CCRL2, CCR3, CCR5 and CCXCR1, are found to form a gene cluster on chromosome 3p.
Gene Symbol
  • CCR1
  • HM145
  • CCR-1
  • MIP1aR
  • RANTES-R
  • CKR-1
  • CD191
  • CMKBR1
  • CC-CKR-1
  • MIP-1alpha-R
  • SCYAR1
  • CMKR1
GPCR ClassA
Host CellsChem-2
Protein TargetCCR1
Target Sub-FamilyChemokine
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P32246 # Receptor for a C-C type chemokine. Binds to MIP-1-alpha, MIP-1-delta, RANTES, and MCP-3 and, less efficiently, to MIP-1- beta or MCP-1 and subsequently transduces a signal by increasing the intracellular calcium ions level. Responsible for affecting stem cell proliferation.
SIZE: 355 amino acids; 41173 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Widely expressed in different hematopoietic cells.
SIMILARITY: SwissProt: P32246 ## Belongs to the G-protein coupled receptor 1 family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceSPECIFICATIONS:
Bmax for [125I]MIP-1alpha binding: 3.3 pmol/mg protein
Kd for [125I] MIP-1alpha binding: ~ 1.8 nM HOST CELLS:
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
Packaging Information
Material Size1 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HTS005M 04053252619908

Documentation

Protocols

Title
Assembly of a Competition Binding Curve

ChemiSCREEN™ CCR1 Membrane Preparation SDS

Title

Safety Data Sheet (SDS) 

ChemiSCREEN™ CCR1 Membrane Preparation Certificates of Analysis

TitleLot Number
ChemiscreenTM MEMBRANE PREPARATION HUMAN RECOMBINANT CCR1 CHEMOKINE RECEPTOR - 2349735 2349735
ChemiscreenTM MEMBRANE PREPARATION HUMAN RECOMBINANT CCR1 CHEMOKINE RECEPTOR - 2052325 2052325
ChemiscreenTM MEMBRANE PREPARATION HUMAN RECOMBINANT CCR1 CHEMOKINE RECEPTOR - 2123198 2123198

References

Reference overviewPub Med ID
Granulocyte-macrophage colony stimulating factor up-regulates CCR1 in human neutrophils.
Cheng, S S, et al.
J. Immunol., 166: 1178-84 (2001)  2001

Show Abstract 		11145699 11145699

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