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14-450M-D CDK1/CyclinB1

Recombinant human CDK1/CyclinB1 complex, also known as CDC and CCNB1, CCNB respectively, full-length with an N-terminal GST-tags on both proteins, expressed in a baculovirus-infected Sf9 cell expression system, for use in enzyme kinetics, screening inhibitors, and selectivity profiling.

CDK1/CyclinB1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-450M-D
250 μg  Also available in 10 μg size (cat#14-450-D)
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-450M-D
Brand Family Upstate
Trade Name
  • Upstate
DescriptionCDK1/CyclinB1
OverviewRecombinant human CDK1/CyclinB1 complex, also known as CDC and CCNB1, CCNB respectively, full-length with an N-terminal GST-tags on both proteins, expressed in a baculovirus-infected Sf9 cell expression system
References
Product Information
Presentation50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol
Quality LevelMQ300
Applications
ApplicationRecombinant human CDK1/CyclinB1 complex, also known as CDC and CCNB1, CCNB respectively, full-length with an N-terminal GST-tags on both proteins, expressed in a baculovirus-infected Sf9 cell expression system, for use in enzyme kinetics, screening inhibitors, and selectivity profiling.
Key Applications
  • Kinase Assay
Biological Information
Concentration0.1 mg/ml
Purity≥75%
SourceSf9 Insect Cells
Specific Activity90 pmol/min/μg
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.
Gene Symbol
  • CDC2
  • CDK1
  • MGC111195
  • DKFZp686L20222
  • CDC28A
Target Sub-FamilyCMGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P06493 # Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
SIZE: 297 amino acids; 34095 Da
SUBUNIT: Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with DLG7. Isoform 2 is unable to complex with cyclin B1 and also fails to bind to the CDK inhibitor p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase.
SUBCELLULAR LOCATION: Nucleus (By similarity).
SIMILARITY: SwissProt: P06493 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
Molecular WeightCDK1: 58 kDa, CyclinB1: 75 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Kinase assay: 10 µl of CDK1/CyclinB1 was mixed with 5 µl Histone H1 substrate (1 mg/ml) and 5 µl water. Reaction was initiated by adding 5 µl of [33P]-ATP cocktail and incubated at 30°C for 15 min. Reaction was read by adding 20 µl to P81 paper and read in the presence of scintillation fluid in a scintillation counter.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for > 6 month at –80°C from date of receipt.
Packaging Information
Material Size250 μg
Material PackageAlso available in 10 μg size (cat#14-450-D)
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-450M-D 04055977276558