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SCT109 BioTracker™ 400 Blue Cytoplasmic Membrane Dye

Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies.

SCT109
1 Kit  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCT109
DescriptionBioTracker™ 400 Blue Cytoplasmic Membrane Dye
OverviewLipophilic carbocyanine dyes are widely used for labeling neurons in tissues by retrograde labeling, and to label membranes in a wide variety of cell types. The dyes are weakly fluorescent in aqueous phase, but become highly fluorescent in lipid bilayers. Staining is highly stable with low toxicity and very little dye transfer in between cells, making the dyes suitable for long-term cell labeling and tracking studies. When live cells are stained, the dyes label plasma membranes and also are taken up into endocytic compartments. Cells can be fixed either before or after staining, although permeabilization affects the staining pattern.

Unlike PKH dyes, BioTracker Cytoplasmic Membrane Dyes do not require a complicated hypoosmotic labeling protocol. They are ready-to-use dye delivery solutions that can be added directly to normal culture media to label suspended or adherent cells in culture.

Spectral Properties
Absorbance: 366nm
Emission: 441nm
Alternate Names
  • Live cell imaging probe
References
Product Information
Components
  • 1. 1 vial of 250µL Cell Labeling Solution (CS224586)
  • 2. 1 vial of 250µL Loading Buffer (CS224587)
Detection methodFluorescence
PresentationLiquid
Applications
ApplicationLive cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies.
Key Applications
  • Cell Based Assays
Application NotesLive cell fluorescent imaging
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceSpectral Properties
Absorbance: 366nm
Emission: 441nm
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore BioTracker 400 Blue Cytoplasmic Membrane Dye at room temp. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Packaging Information
Material Size1 Kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCT109 04054839566073